Comparative histomorphological assessment of the osteoinductive capacity of a nanofibrillated cellulose-based composite and autologous blood clot
Tuleubayev B. Kamyshanskiy Y. Saginova D. Akhmetova S. Vinokurov V. Koshanova A. Kossilova Y.
October 2024John Wiley and Sons Inc
Journal of Experimental Orthopaedics
2024#11Issue 4
Purpose: The present study aimed to evaluate and compare the effect of nanofibrillated cellulose (NFC)-based composite with dicalcium phosphate dihydrate and an autologous blood clot (ABC) on the formation of new bone tissue in vivo by histological and histomorphometric assessment. Materials and Methods: A total of 72 rats with created femoral defects (2 mm) were used. The rats were divided into three groups: (1) with filling of the defect with an ABC, (2) NFC-1—with filling of both the cortical plate and intramedullary space in the defect area, and (3) NFC-2—with filling of only the intramedullary space in the defect area. Histological and histomorphometric analysis was performed to assess the healing of the bone defect after 14, 30 and 60 days. Results: Complete closure of the cortical plate defect was detected in the NFC-2 group on Day 30 (p < 0.0001). Moreover, in both NFC groups on the 30th and 60th days, ongoing osteogenesis was observed, characterized by a large volume of newly formed circular pattern bone tissue in the intramedullary space. Сonclusion: This study demonstrated that the NFC-based composite, which is located below the level of the cortical plate, tamponing only the intramedullary space (NFC-2), improves bone tissue repair at the site of a bone defect of the cortical plate and has the potential of prolonged osteoinductivity. Level of Evidence: Not applicable.
autologous blood clot , bone defect , bone regeneration , composite , nanocellulose
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Department of Surgical Diseases, Karaganda Medical University, Karaganda, Kazakhstan
Pathology Unit of the University Clinic, Karaganda Medical University, Karaganda, Kazakhstan
Center for Applied Scientific Research, National Scientific Center of Traumatology and Orthopaedics Named after Academician N.D. Batpenov, Astana, Kazakhstan
Department of Clinical Immunology, Karaganda Medical University, Karaganda, Kazakhstan
Department of Physical and Colloidal Chemistry, Federal State Autonomous Educational Institution of Higher Education Gubkin Russian State University of Oil and Gas, National Research University, Moscow, Russian Federation
Department of Surgical Diseases
Pathology Unit of the University Clinic
Center for Applied Scientific Research
Department of Clinical Immunology
Department of Physical and Colloidal Chemistry
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