A histologic and histomorphometric analysis of bone tissue regeneration with perforated bone allograft in rabbit femur defect
Tuleubaev B.E. Kamyshansky E.K. Azimovna S.D. Tashmetov E.R. Koshanova A.A.
2021Open Access Macedonian Journal of Medical Sciences
Open Access Macedonian Journal of Medical Sciences
2021#9Issue A12 - 18 pp.
AIM: The aim of this study is to provide a comparative histopathological evaluation of the regeneration of bone defect filling with perforated antibiotic-impregnated bone allograft. MATERIALS AND METHODS: Seventy-two healthy rabbits (24 rabbits in each group) were used for this study. Bone defects (3-mm diameter, 10-mm depth) were created in the femur. Human femoral head prepared according to the Marburg bone bank system was used as a bone allograft. The control group did not receive any filling. The experimental groups were as follows: Group 1 – the defects were filled with bone allografts and Group 2 – Perforated gentamycin-impregnated bone allografts. The animals were euthanized after 14, 30, and 60 days. Evaluations consisted of histology at 14-, 30-, and 60-days post-surgery. RESULTS: A mature bone formation in the group without a bone allograft occurred after 30 days and the group with an allograft after 14 days. In the groups with an allograft, a bone marrow defect was noted as complete closure after 30 days. Histomorphometric analysis showed that in the group with an antibiotic-impregnated bone, allograft leads to increased resorption of the allograft in the intramedullary space compared to group without antibiotic. CONCLUSION: We believe that a perforated allograft as a result of clinical trials may be obvious and economically affordable in the treatment of bone defects. The use of gentamycin-impregnated bone allografts may be of value in the prevention and treatment of bone infections.
Bone allograft , Bone defect , Bone regeneration , Marburg bone bank
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Department of Surgical Diseases, Karaganda Medical University, Karaganda, Kazakhstan
Laboratory and Pathological Unit, Clinic of Karaganda Medical University, Karaganda, Kazakhstan
Department of Surgical Diseases
Laboratory and Pathological Unit
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