HPLC-ESI-QTOF-MS/MS ANALYSIS OF ARTEMISIA CINA LEAVES AQUEOUS EXTRACT AND FLAVONOID ISOLATION USING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
Tshunungwa S. Alsharif B. Bekezhanova T. Bazhykova K.B. Kukula-Koch W. Boylan F.
2024Polish Pharmaceutical Society
Acta Poloniae Pharmaceutica - Drug Research
2024#81Issue 2319 - 330 pp.
Artemisia cina Berg ex Poljakov, a shrubby plant endemic to the semi-arid areas of Kazakhstan, has as a long history of being used in traditional medicine as an antitumour, antifungal, and anthelmintic agent. In a preliminary study carried out on different methodologies to produce aqueous extracts of A. cina, it was seen that a simple aqueous extract of A. cina (as in an infusion of the plant) had strong anti-SARS-CoV-2 activity. In the current study, profiling of this aqueous extract was performed using Liquid Chromatography-Electrospray Ionization-Quadrupole-Time-of-Flight Mass Spectrometry (LC-ESI-Q-TOF-MS) while High-Speed Countercurrent Chromatography (HSCCC) and a Sephadex LH-20 column were utilised to isolate and purify the extract in ethyl acetate obtained from the original infusion. Nuclear Magnetic Resonance (NMR) was used for structural elucidation. The flavonoid spinacetin 7-O-glucoside was found to be one of the main compounds present in the A. cina infusion. Flavonoids and artemisinin identified by LC-MS in this infusion are hypothesised to be the key compounds behind its anti-SARSCoV-2 activity, however this needs to be further tested and explored.
aqueous infusion , Artemisia cina , artemisinin , flavonoids , HSCCC , spinacetin-7-O-glucoside
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School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 02, Ireland
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia
Department of Engineering Disciplines and Good Practices, Asfendiyarov Kazakh National Medical University, Almaty, 050000, Kazakhstan
Department of Chemistry and Technology of Organic Substances, Natural Compounds and Polymers, Al-Farabi Kazakh National University, Almaty, 050000, Kazakhstan
Department of Pharmacognosy, Medical University of Lublin, Chodzki 1, Lublin, 20-093, Poland
Trinity Natural Products Research Centre, NatPro Centre, Trinity College Dublin, Dublin, 02, Ireland
School of Pharmacy and Pharmaceutical Sciences
Department of Pharmaceutical Sciences
Department of Engineering Disciplines and Good Practices
Department of Chemistry and Technology of Organic Substances
Department of Pharmacognosy
Trinity Natural Products Research Centre
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