Determination of Giardia duodenalis (Metamonada: Hexamitidae) genotypes in water buffalo
Tokseiit Y. Albahadly W.K.Y. Radhi H. Abduljabbar M.H. Ahmed M. Alwash S.W. Yousif Z.S. Obeed A.N. Saule J.
April 2023University of Guilan
Caspian Journal of Environmental Sciences
2023#21Issue 2389 - 393 pp.
Giardia duodenalis is considered as one of the important common protozoa between humans and animals in the intestine, which has eight groups (A-H) in different hosts. Studies have shown that the assemblages A, B, and E can infect livestock. In this study, the prevalence and genotype of Giardia duodenalis was determined by polymerase chain reaction of ssu-rRNA gene and by performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on glutamate dehydrogenase gene of the parasite in water buffalo (n = 60) from Iraq. Based on the results, prevalence of G. duodenalis infection in water buffalo was estimated 25 % (n = 15), and according to age groups, higher infection group was 40% at ≤ 6 months, followed by > 6-12 months, 1-2 years, >2 years which showed 26.6%, 13.3% and 20.1%, respectively. The result of distribution of genotypes of G. duodenalis showed that assemblages AI and E were recorded in water buffalo isolates by 60 % and 40 %. Infection of assemblage AI was reported in buffaloes under 12 months. The present study determined that G. duodenalis is highly prevalent in water buffalo, and is involved in creating a zoonotic disease for giardiasis in Iraq. Due to the direct relationship between food and public health, as well as the influence of geographic and host conditions on the spread and pathogenicity, it is necessary to further investigate different genotypes and their common aspects between humans and livestock.
Genotype , Giardia duodenalis , glutamate dehydrogenase , Iraq , ssu-rRNA , water buffalo
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Department of Philosophy, M. Auezov South Kazakhstan University, Tauke Khan street 5,, Shymkent, Kazakhstan
University of Al-Ameed, College of Pharmacy, Iraq
College of MLT, University of Ahl Al Bayt, Kerbala, Iraq
Department of Pharmacy, Al-Noor University College, Nineveh, Iraq
Medical Technical College, Al-Farahidi University, Iraq
Medical Laboratory Techniques Department, Al-Mustaqbal University College, Babylon, Hillah, 51001, Iraq
Medical Laboratory Techniques Department, Mazaya University College, Iraq
Al-Hadi University College, Baghdad, 10011, Iraq
Institute of Natural Sciences and Geography, Abai Kazakh National pedagogical university, Almaty, Kazakhstan
Department of Philosophy
University of Al-Ameed
College of MLT
Department of Pharmacy
Medical Technical College
Medical Laboratory Techniques Department
Medical Laboratory Techniques Department
Al-Hadi University College
Institute of Natural Sciences and Geography
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