Decellularization of bone tissue in a supercritical carbon dioxide environment
Tokpayev R.R. Shreider E.R. Ibraimov Z.T. Shalakhmetova T.M. Nauryzbayev M.K.
25 December 2023al-Farabi Kazakh State National University
International Journal of Biology and Chemistry
2023#16Issue 216 - 26 pp.
The use of pig bone tissue as a graft makes it possible to obtain high-quality, relatively cheap and biocompatible ECM. This is since the mineral composition of pork bone is most similar to the mineral composition of human bone. Decellularization in SC-CO2 is a promising direction, since carbon dioxide easily diffuses into the depth of the cell and is a good solvent for lipids, it is non-flammable, non-toxic and chemically non-aggressive. In this paper, the ECM in SC-CO2 was obtained in three ways: 1) in dynamic mode with large SC-CO2 flows; 2) in static and dynamic modes with large SC-CO2 flows; 3) in static and dynamic modes with large SC-CO2 flows, with preliminary exposure in ethyl alcohol as a co-solvent. According to the histological examination, the removal of ICC by the first method is 55%. The use of a co-solvent before starting the decellularization process increases the percentage of ICC removal to 98%, which allows the use of ECM as a transplant.
biomaterials , carbon dioxide , decellularization , extracellular matrix , supercritical extraction , tissue engineering , transplantation , xenografts
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Center of physico-chemical methods of research and analysis, al-Farabi Kazakh National University, Almaty, Kazakhstan
School of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, Kazakhstan
Center of physico-chemical methods of research and analysis
School of Biology and Biotechnology
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