Germination of chamomile (Matricaria recutita) seeds during cryopreservation using PVS2 cryoprotectant

Tleukenova S. Ramazanov A. Ishmuratova M. Mussina R. Atikeeva S. Nurkenova A. Zhumadilov S. Shapatova G. Sarsembaeva A.
June 2022 Gaurav Publications

Research on Crops
2022 #23 Issue 2 466 - 472 pp.

Preservation of plant world biodiversity, especially medicinal species, is one of the main tasks of modern science. Seeds are the most optimal form of genetic material storage, as samples require little space and remain viable for a long period. There are several methods for storing plant seed. One of the most inexpensive and effective methods is cryopreservation. The present study was conducted at with an objective study the seeds of Matricaria recutita for cryopreservation and viability of seeds. The seed material was frozen in liquid nitrogen (Dewar vials) using cryoprotectant PVS2. The assessment of seed viability was conducted in laboratorial conditions on Petri dishes. The results of the study showed that cryopreservation of Matricaria recutita seeds is better to carry out without the use of PVS2 cryoprotectant, since the germination of seeds was lower than the control (56%) (seed freezing without cryoprotective substances). Seeds’ sowing after cryopreservation is recommended in two versions, immediately after defrosting and also with a delay in sowing, which makes it possible to transport thawed seeds to outlying regions.

Cryopreservation , cryoprotectant , Matricaria recutita , seed germination

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Laboratory of Biotechnology and Ecomonitoring, E. A. Buketov Karaganda University, 28 University Street, Karaganda, 100028, Kazakhstan
Design, Service and Tourism department, Turan University, 29 Ykylas Dukenuly Street, Nur-Sultan, 010000, Kazakhstan
Morphology and Physiology department, Karaganda Medical University, 40 Gogol Street, Karaganda, 100000, Kazakhstan

Laboratory of Biotechnology and Ecomonitoring
Design
Morphology and Physiology department

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