Component Composition and Antimicrobial Activity of CO2Extract of Portulaca oleracea, Growing in the Territory of Kazakhstan
Tleubayeva M.I. Datkhayev U.M. Alimzhanova M. Ishmuratova M.Y. Korotetskaya N.V. Abdullabekova R.M. Flisyuk E.V. Gemejiyeva N.G.
2021Hindawi Limited
Scientific World Journal
2021#2021
In the medicine of many countries, the use of herbal healing agents included a significant contribution to improving human health and well-being. Many antibiotics have been widely used to treat infectious diseases caused by various pathogenic bacteria. However, increased multidrug resistance has led to increased severity of diseases caused by bacterial pathogens. Bacteria remain the main causative agents of diseases that cause human death, even in the present day. This cause prompted scientists to investigate alternative new molecules against bacterial strains. The significant interest for the study is Portulaca oleracea L. (family Portulacaceae), a widespread annual plant used in folk medicine. Thus, the production and study of CO2 extract of Portulaca oleracea is an actual problem. Methods. Raw materials were collected from Almaty and Zhambyl regions (Southeast and South Kazakhstan) in phase flowering. Portulaca oleracea herbs CO2 extract was obtained by subcritical carbon dioxide extraction (installation of carbon dioxide flow-through extraction- 5L). The Wiley 7th edition and NIST02 library were used to identify the mass spectra obtained. The antimicrobial activity study was conducted by the micromethod of serial dilution and disco-diffuse method. Standard test strains of microorganisms were used: Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538-P, Candida albicans ATCC 10231, and Escherichia coli ATCC 8739. Results. The use of carbon dioxide extraction (further CO2 extract) is a promising direction of obtaining total medicinal substances containing biologically active substances, from fractions of volatile esters of various composition and functional purpose until a fraction of fatty acids and fat-soluble vitamins. In the current study, we obtained CO2 extract at subcritical conditions from aboveground organs of Portulaca oleracea and investigated the component composition for the first time. From 41 to 66 components were identified in the composition of Portulaca oleraceas CO2 extract. Studies of antimicrobial activity showed that CO2 extract of Portulaca oleracea had the expressed effect against clinically significant microorganisms such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans. Conclusions. This study showed that CO2 extract of Portulaca oleraceas raw material contained biological active compounds exhibiting a significant antimicrobial effect.
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Department of Organization, Management and Economics of Pharmacy and Clinical Pharmacy, School of Pharmacy, Asfendiyarov Kazakh National Medical University, St.Tole Bi, 88, Almaty, 050000, Kazakhstan
Department of Analytical, Colloidal Chemistry and Technology of Rare Elements, Al-Farabi Kazakh National University, Al-Farabi Ave, 71, Almaty, 050040, Kazakhstan
Department of Botany, E. Buketov Karaganda University, St. University, 28, Karaganda, 100028, Kazakhstan
Test Facility Management, JSC Scientific Center for Antiinfectious Drags, Al-Farabi Ave, 75 A, Almaty, 050060, Kazakhstan
Department of Pharmaceutical Disciplines and Chemistry, Medical University of Karaganda, St. Gogol, 40, Karaganda, 100008, Kazakhstan
Department Technology of Drags Forms, Saint-Petersburg State University of Chemical and Pharmaceuticals, St. Popova, 14, St. Petersburg, 197376, Russian Federation
Laboratory of Plant Resources, Institute of Botany and Phyto-Introductions, St. Timiryazev, 36 D, Almaty, 050040, Kazakhstan
Department of Organization
Department of Analytical
Department of Botany
Test Facility Management
Department of Pharmaceutical Disciplines and Chemistry
Department Technology of Drags Forms
Laboratory of Plant Resources
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