Comprehensive Quality Assessment of Brassica napus L. Seeds via HPTLC, LC-QToF, and Anatomical Investigation
Tileuberdi N. Katragunta K. Adams S.J. Aldana-Mejía J.A. Omarbekova A. Avula B. Khan I.A. Turgumbayeva A. Ross S.A.
July 2024Multidisciplinary Digital Publishing Institute (MDPI)
Molecules
2024#29Issue 13
The Brassicaceae family, commonly referred to as cruciferous plants, is globally cultivated and consumed, with the Brassica genus being particularly renowned for its functional components. These vegetables are rich sources of nutrients and health-promoting phytochemicals, garnering increased attention in recent years. This study presents a comprehensive microscopic, chromatographic, and spectroscopic characterization of Brassica napus L. seeds from Kazakhstan aimed at elucidating their morphological features and chemical composition. Microscopic analysis revealed distinct localization of flavonoids, total lipids, and alkaloids. High-performance thin-layer chromatography (HPTLC) analysis of seed extracts demonstrated a complex chemical profile with significant quantities of non-polar compounds in the hexane extracts. Additionally, methanolic extracts revealed the presence of diverse chemical compounds, including alkaloids, flavonoids, and glucosinolates. The chemical composition exhibited varietal differences across different Brassica species, with B. napus L. seeds showing higher concentrations of bioactive compounds. Furthermore, liquid chromatography–quadrupole time-of-flight mass spectrometry (LC-QToF-MS) analysis provided insights into the chemical composition, with sinapine isomers, feruloyl, and sinapoyl choline derivatives as major compounds in the seeds. This study contributes to a better understanding of the chemical diversity and quality control methods’ approximations of B. napus L. seeds, highlighting their importance in functional food and nutraceutical applications.
Brassicaceae , flavonoids , histochemistry , microscopy , phytochemistry , quality control
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Higher School of Medicine, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, 38677, MS, United States
School of Pharmacy, Asfendiyarov Kazakh National Medical University, Almaty, 050012, Kazakhstan
Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, Oxford, 38677, MS, United States
Higher School of Medicine
National Center for Natural Products Research
School of Pharmacy
Division of Pharmacognosy
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