INVESTIGATION OF BIOCHEMICAL PROPERTIES, ANTIMICROBIAL, ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF THE RHAMNUS CATHARTICA L. EXTRACTS COLLECTED FROM KAZAKHSTAN

KAZAKİSTAN’DAN TOPLANAN RHAMNUS CATHARTİCA L. EKSTRAKTLARININ BİYOKİMYASAL ÖZELLİKLERİ, ANTİMIKROBİYAL, ANTİOKSİDAN VE ANTİPROLİFERATİF AKTİVİTELERİNİN ARAŞTIRILMASI
Tileshnova A.S. Zura Y. Tunç T. Özpinar H.
19 September 2025 University of Ankara

Ankara Universitesi Eczacilik Fakultesi Dergisi
2025 #49 Issue 3 743 - 755 pp.

Objectives: This research evaluated the biochemical composition of hexane, ethyl acetate, and ethanol extracts and analyzed the antimicrobial, antioxidant and antiproliferative activities of the cortex, leaf, and fruit of the Rhamnus cathartica L. plant from Almaty, Kazakhstan. Material and Method: The GC-MS method was used to evaluate the chemicals of Rhamnus cathartica L. cortex, leaf, and fruit extracts. The microdilution technique was used to evaluate the MIC values of plant extracts against pathogenic microorganisms. DPPH, FRAP, and Total Flavonoid Content were evaluated for antioxidant activity. The antiproliferative effect of the extracts on MCF-7, A549, C6 and WI-38 cells was measured by XTT assay. Result and Discussion: According to GC-MS results, Trans-2-Decenal had the highest proportion at 2.88%. Eicosane is second at 1.71%. DPPH, FRAP and flavonoid values showed that the fruit ethyl acetate extract (F-EtAc) had the strongest antioxidant activity. F-EtAc extract had the strongest anticancer activity according to the selectivity index table (WI-38/MCF-7: 12.67, WI-38/C6: 31.38, WI-38/A549: 8.55). All plant extracts had high MIC values against Staphylococcus aureus based on reference source values. The MIC value of cortex and leaf in ethyl acetate was 0.078 mg/ml, cortex and leaf extracts had strong antimicrobial activity (0.312 mg/ml) for Bacillus cereus, while fruits had weakly effective MIC values (Significant=MIC <0.1 mg/ml). According to the results of this study, it can be said that Rhamnus cathartica L. plant extracts have an active content in terms of biological properties.

Antimicrobial , antioxidant , antiproliferative , Rhamnus cathartica

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Department of Biodiversity and. Bioresources, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Sivas Cumhuriyet University, Sivas, 58140, Turkey
Department of Pharmaceutical Botany, Faculty of Pharmacy, Sivas Cumhuriyet University, Sivas, 58140, Turkey

Department of Biodiversity and. Bioresources
Department of Pharmaceutical Microbiology
Department of Pharmaceutical Botany

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