Influence of Cold Stress on Physiological and Phytochemical Characteristics and Secondary Metabolite Accumulation in Microclones of Juglans regia L.
Terletskaya N.V. Shadenova E.A. Litvinenko Y.A. Ashimuly K. Erbay M. Mamirova A. Nazarova I. Meduntseva N.D. Kudrina N.O. Korbozova N.K. Djangalina E.D.
May 2024Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences
2024#25Issue 9
The current study investigated the impact of cold stress on the morphological, physiological, and phytochemical properties of Juglans regia L. (J. regia) using in vitro microclone cultures. The study revealed significant stress-induced changes in the production of secondary antioxidant metabolites. According to gas chromatography–mass spectrometry (GC–MS) analyses, the stress conditions profoundly altered the metabolism of J. regia microclones. Although the overall spectrum of metabolites was reduced, the production of key secondary antioxidant metabolites significantly increased. Notably, there was a sevenfold (7×) increase in juglone concentration. These findings are crucial for advancing walnut metabolomics and enhancing our understanding of plant responses to abiotic stress factors. Additionally, study results aid in identifying the role of individual metabolites in these processes, which is essential for developing strategies to improve plant resilience and tolerance to adverse conditions.
anatomy , cold stress , Juglans regia , metabolome , microclones
Text of the article Перейти на текст статьи
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty, 050040, Kazakhstan
Institute of Genetic and Physiology, Al-Farabi 93, Almaty, 050040, Kazakhstan
Faculty of Chemistry, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty, 050040, Kazakhstan
Faculty of Biology and Biotechnology
Institute of Genetic and Physiology
Faculty of Chemistry
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026