Mouse Brain Lymphatic Vessels
Tamadon A. Afshar A. Mussin N.M. Zhilisbayeva K.R. Kurmanalina M.A. Baspakova A. Aringazina R.A. Mahdipour M. Tanideh N. Khoradmehr A. Najarasl M. Taheri P.
2025American Chemical Society
ACS Chemical Neuroscience
2025#16Issue 234492 - 4501 pp.
This study provides evidence of lymphatic vessels in the mouse brain, specifically in the cortex, thalamus, and hippocampus. Using confocal microscopy, Western blotting, and real-time PCR, lymphatic vessels were identified by the expression of Lyve1, Prox1, and VEGFC, alongside endothelial markers CD31 and CD34. Paraffin-embedded brain slices from wild-type Balb/C mice were stained with antibodies against Lyve1, CD31, and CD34, revealing lymphatic vessels through 2D and 3D imaging. A coiled 3D structure of lymphatic vessels was observed in the hippocampus, indicating a complex drainage network. Western blot and real-time PCR analyses confirmed the presence of lymphatic and endothelial markers at the protein and mRNA levels. These findings demonstrate a lymphatic system extending from the meninges into deeper brain regions, offering insights into immune and waste clearance pathways in the central nervous system and potential therapeutic targets for neurological disorders.
brain immunology , hippocampus , lymphatic system , lymphatic vessels , meningeal lymphatics , neuroimmunology
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Department of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Aktobe, 030012, Kazakhstan
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, 71348-14336, Iran
Department of General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, 030012, Kazakhstan
Department of Languages, West Kazakhstan Marat Ospanov Medical University, Aktobe, 030012, Kazakhstan
Department of Therapeutic and Prosthetic Dentistry, West Kazakhstan Marat Ospanov Medical University, Aktobe, 030012, Kazakhstan
Department of Epidemiology, West Kazakhstan Marat Ospanov Medical University, Aktobe, 030012, Kazakhstan
Department of Internal Medicine No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, 030012, Kazakhstan
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, 51656-65931, Iran
Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, 51656-65931, Iran
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, 71348-53185, Iran
The Persian Gulf Marine Biotechnology Research Center, the Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, 75146-33196, Iran
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Acecr, Tehran, 16656-59911, Iran
Department of Natural Sciences
Stem Cells Technology Research Center
Department of General Surgery
Department of Languages
Department of Therapeutic and Prosthetic Dentistry
Department of Epidemiology
Department of Internal Medicine No. 1
Stem Cell Research Center
Department of Applied Cell Sciences
Department of Pharmacology
The Persian Gulf Marine Biotechnology Research Center
Department of Stem Cells and Developmental Biology
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