BIOCHEMICAL CHARACTERIZATION, ANTI-PROLIFERATIVE AND CYTOTOXICITY EFFECT OF PURIFIED L-ASPARAGINASE, AN ANTI-LEUKEMIA ENZYME ISOLATED FROM NEW BACTERIA Myroides Gitamensis
Talluri V.S.S.L.P. Lanka S.S. Mutaliyeva B. Sharipova A. Suigenbayeva A. Tleuova A.
April-June 2022Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma
Rasayan Journal of Chemistry
2022#15Issue 2786 - 791 pp.
L-asparaginase (L-asp) is used in the treatment of acute lymphocytic leukemia (ALL). L-asp catalyzes the L-asp into ammonia and L-aspartic acid through deamination. The formed ammonia finds to be toxic to the organism. Therefore, the search for less cytotoxic anti-proliferative L-asp is of great importance. Because of its costeffectiveness, consistency, and easiness of process control, microbial L-asparaginase is chosen over plant or animal enzymes. L-asp enzyme which was studied in the article was isolated from a newly discovered bacteria Myroides gitamensis. The biochemical characteristics and antiproliferative, cytotoxic effect of purified L-asp were studied in order to develop safer biochemically stable anti-leukemia medication. To provide comprehensive information on the characteristics of the isolated L-asparaginase enzyme analytical tools were utilized, including cancer cell lines, brine shrimps, enzyme reactions etc. MOLT-4 and K-562 cell lines showed an IC50 value of 91.41 IU/mL and 77.42 IU/mL, respectively. Cytotoxicity activity of L-asp on Artemia salina showed that at 24 hours (LC50 value of 217.0 µg/mL) and toxic at 48 hours (LC50 value of 126.4µg/ml).
Antiproliferative Effect , Biochemical Characterization , Cytotoxic Activity , Enzyme , L-asparaginase , Leukemia
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Department of Biotechnology, Gandhi Institute of Technology and Management (GITAM) University, Visakhapatnam, 530045, India
National University of Singapore, Singapore, 117546, Singapore
Department of Biotechnology, M.Auezov South Kazakhstan University, Shymkent, 160012, Kazakhstan
Department of Metallurgy and mineral processing, Satbayev University, Almaty, 050013, Kazakhstan
Department of Chemical Technology of Inorganic Substances, M.Auezov South Kazakhstan University, Shymkent, 160012, Kazakhstan
Department of Biotechnology
National University of Singapore
Department of Biotechnology
Department of Metallurgy and mineral processing
Department of Chemical Technology of Inorganic Substances
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