Ultralow Limit Detection of Soluble HER2 Biomarker in Serum with a Fiber-Optic Ball-Tip Resonator Assisted by a Tilted FBG
Sypabekova M. Amantayeva A. Vangelista L. González-Vila A. Caucheteur C. Tosi D.
17 August 2022American Chemical Society
ACS Measurement Science Au
2022#2Issue 4309 - 316 pp.
An optical-fiber biosensor has been developed for the detection of the breast cancer biomarker soluble human epidermal growth factor receptor-2 (sHER2). The sensor was fabricated by combining a tilted fiber Bragg grating (TFBG) with a ball resonator, allowing us to achieve an excellent sensitivity compared to other optical-fiber-based sensors. The sensor exhibits a resonance comb excited by the TFBG and the spectral profile of the ball resonator. The detection of sHER2 at extremely low concentrations was carried out by tracking the amplitude change of selected resonances. The therapeutic anti-HER2 monoclonal antibody Trastuzumab has been used to functionalize the biosensor with silane surface chemistry. The sensor features a sensitivity of 4034 dB/RIU with a limit of detection (LoD) in buffer and in a 1/10 diluted serum of 151.5 ag/mL and 3.7 pg/mL, respectively. At relatively high protein concentrations (64 ng/mL) binding to sHER (7.36 dB) as compared to control proteins (below 0.7 dB) attested the high specificity of sHER2 detection.
cancer biomarker detection , cancer diagnostic , fiber Bragg grating , HER2 detection , human epidermal growth factor-2 (HER2) , low-limit biosensor , optical-fiber biosensors
Text of the article Перейти на текст статьи
Nazarbayev University School of Medicine, 53 Kabanbay Batyr Avenue, Nur-Sultan, 010000, Kazakhstan
Nazarbayev University School of Engineering and Digital Sciences, 53 Kabanbay Batyr Avenue, Nur-Sultan, 010000, Kazakhstan
Baylor Research and Innovative Collaborative, Baylor University, 100 Research Pkwy, Waco, 76704, TX, United States
Electromagnetism and Telecommunication Department, University of Mons, Boulevard Dolez 31, Mons, 7000, Belgium
National Laboratory Astana, Laboratory of Biosensors and Bioinstruments, Nur-Sultan, 010000, Kazakhstan
Nazarbayev University School of Medicine
Nazarbayev University School of Engineering and Digital Sciences
Baylor Research and Innovative Collaborative
Electromagnetism and Telecommunication Department
National Laboratory Astana
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026