Stress-Induced Secondary Metabolite Profiling in Cistanche deserticola Callus Cultures: Insights from GC-MS and HPLC-MS Analysis

Sutula M. Gubaidullin N. Rakhimzhanova A. Manabayeva S.
July 2025 Multidisciplinary Digital Publishing Institute (MDPI)

International Journal of Molecular Sciences
2025 #26 Issue 13

Throughout human history, wild plant resources have played an invaluable role, serving as critical sources of food, medicine, and industrial materials. This study examined the callus cultures of Cistanche deserticola Y.C. Ma, a medicinal desert plant, by subjecting them to abiotic stress under controlled in vitro conditions. The secondary metabolite profiles were then analyzed using GC-MS and qTOF-UHPLC-MS. The GC-MS analysis revealed several bioactive compounds of pharmaceutical interest, such as γ-sitosterol and homovanillyl alcohol. PhGs, including echinacoside and salidroside, were quantified for the first time across 16 callus samples exposed to various stress treatments. The application of 0.1% Na₂CO₃ for 50 days resulted in the highest accumulation of echinacoside (13,378.9 µg/mL), and heavy metal stress notably increased salidroside levels to 27.0 µg/mL. There was a clear correlation between callus pigmentation and metabolic activity: orange and white calli produced significantly more PhGs than dark calli. These results suggest that C. deserticola callus cultures could be a sustainable, controllable platform for producing high-value secondary metabolites. This reinforces the importance of wild plant resources in modern science and industry.

C. deserticola , callus culture , GC-MS , PhGs , qTOF-UHPLC-MS , secondary metabolites

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Plant Genetic Engineering Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
Faculty of Natural Sciences, L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan

Plant Genetic Engineering Laboratory
Faculty of Natural Sciences

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