Identification of mushroom and murine tyrosinase inhibitors from Achillea biebersteinii Afan. Extract
Strzępek-Gomółka M. Gaweł-Bęben K. Angelis A. Antosiewicz B. Sakipova Z. Kozhanova K. Głowniak K. Kukula-Koch W.
2 February 2021MDPI AG
Molecules
2021#26Issue 4
Growing scientific evidence indicates that Achillea biebersteinii is a valuable source of active ingredients with potential cosmetic applications. However, the data on its composition and pharmacological properties are still insufficient. This study aims to optimize the extraction procedure of the plant material, evaluate its phytochemical composition, and compare anti-tyrosinase potential of A. biebersteinii extracts obtained by various methods. In order to identify compounds responsible for the tyrosinase inhibitory activity of A. biebersteinii, the most active anti-tyrosinase extract was fractionated by column chromatography. The fractions were examined for their skin lightening potential by mushroom and murine tyrosinase inhibitory assays and melanin release assay. HPLC-ESI-Q-TOF-MS/MS analysis of the total extract revealed the presence of several phenolic acids, flavonoids, flavonoid glucosides, and carboxylic acid. Among them, fraxetin-8-O-glucoside, quercetin-O-glucopyranose, schaftoside/isoschaftoside, gmelinin B, 1,3-dicaffeoylquinic acid (1,3- DCQA), and ferulic acid were found in the fractions with the highest skin lightening potential. Based on obtained qualitative and quantitative analysis of the fractions, it was assumed that the caffeoylquinic acid derivatives and dicaffeoylquinic acid derivatives are more likely responsible for mushroom tyrosinase inhibitory activity of A. biebersteinii extracts and fractions. Ferulic acid was proposed as the most active murine tyrosinase inhibitor, responsible also for the reduced melanin release from B16F10 murine melanoma cells.
Achillea biebersteinii , Antioxidant properties , HPLC-MS analysis , Melanin release , Murine tyrosinase , Mushroom tyrosinase , Tyrosinase inhibition
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Department of Cosmetology, University of Information Technology and Management in Rzeszów, Sucharskiego 2, Rzeszów, 35-225, Poland
Laboratory of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimioupoli Zografou, Athens, 15771, Greece
School of Pharmacy, Kazakh National Medical University Named after S.D. Asfendiyarov (KazNMU), 88 Tole Bi Street, Almaty, 050012, Kazakhstan
Department of Pharmacognosy, Medical University in Lublin, Chodźki 1, Lublin, 20-093, Poland
Department of Cosmetology
Laboratory of Pharmacognosy and Natural Products Chemistry
School of Pharmacy
Department of Pharmacognosy
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