Intramuscular mRNA BNT162b2 vaccine against SARS-CoV-2 induces neutralizing salivary IgA
Stolovich-Rain M. Kumari S. Friedman A. Kirillov S. Socol Y. Billan M. Pal R.R. Das K. Golding P. Oiknine-Djian E. Sirhan S. Bejerano Sagie M. Cohen-Kfir E. Gold N. Fahoum J. Kumar M. Elgrably-Weiss M. Zhou B. Ravins M. Gatt Y.E. Bhattacharya S. Zelig O. Wiener R. Wolf D.G. Elinav H. Strahilevitz J. Padawer D. Baraz L. Rouvinski A.
30 January 2023Frontiers Media SA
Frontiers in Immunology
2023#13
Intramuscularly administered vaccines stimulate robust serum neutralizing antibodies, yet they are often less competent in eliciting sustainable “sterilizing immunity” at the mucosal level. Our study uncovers a strong temporary neutralizing mucosal component of immunity, emanating from intramuscular administration of an mRNA vaccine. We show that saliva of BNT162b2 vaccinees contains temporary IgA targeting the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus-2 spike protein and demonstrate that these IgAs mediate neutralization. RBD-targeting IgAs were found to associate with the secretory component, indicating their bona fide transcytotic origin and their polymeric multivalent nature. The mechanistic understanding of the high neutralizing activity provided by mucosal IgA, acting at the first line of defense, will advance vaccination design and surveillance principles and may point to novel treatment approaches and new routes of vaccine administration and boosting. Copyright
BNT162b2 vaccine , mucosal immunity , SARS-CoV-2 neutralizing Abs , secretory component , secretory IgA
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Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel
The Kuvin Center for the Study of Infectious and Tropical Diseases, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel
Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel
Department of Biochemistry, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel
National Center for Biotechnology, Astana, Kazakhstan
Department of General Biology and Genomics, L.N. Gumilyov Eurasian National University, Astana, Kazakhstan
Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel
Blood Bank, Hadassah Hebrew University Medical Center, Jerusalem, Israel
Lautenberg Centre for Immunology and Cancer Research, The Institute for Medical Research Israel-Canada (IMRIC), Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel
Department of Clinical Microbiology and Infectious Diseases, Hadassah AIDS Center, Hadassah Hebrew University Medical Center, Jerusalem, Israel
Department of Clinical Microbiology and Infectious Diseases, Hadassah Hebrew University Medical Center, Jerusalem, Israel
Institute of Pulmonary Medicine, Hadassah Medical Center, Affiliated to the Faculty of Medicine, Hebrew University Jerusalem, Jerusalem, Israel
Department of Internal Medicine D, Hadassah Medical Center, affiliated to the Faculty of Medicine, Hebrew University, Jerusalem, Israel
Hadassah Academic College Jerusalem, Jerusalem, Israel
School of Biological Sciences, Indian Association for the Cultivation of Science, Kolkata, India
Hadassah Hebrew University Medical Center, Jerusalem, Israel
Department of Microbiology and Molecular Genetics
The Kuvin Center for the Study of Infectious and Tropical Diseases
Department of Developmental Biology and Cancer Research
Department of Biochemistry
National Center for Biotechnology
Department of General Biology and Genomics
Clinical Virology Unit
Blood Bank
Lautenberg Centre for Immunology and Cancer Research
Department of Clinical Microbiology and Infectious Diseases
Department of Clinical Microbiology and Infectious Diseases
Institute of Pulmonary Medicine
Department of Internal Medicine D
Hadassah Academic College Jerusalem
School of Biological Sciences
Hadassah Hebrew University Medical Center
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