Endogenous ureides are employed as a carbon source in Arabidopsis plants exposed to carbon starvation conditions
Soltabayeva A. Kurmanbayeva A. Bekturova A. Oshanova D. Nurbekova Z. Srivastava S. Standing D. Zdunek-Zastocka E. Sagi M.
July 2024Elsevier Ireland Ltd
Plant Science
2024#344
Ureides, the degraded products of purine catabolism in Arabidopsis, have been shown to act as antioxidant and nitrogen sources. Herein we elucidate purine degraded metabolites as a carbon source using the Arabidopsis Atxdh1, Ataln, and Ataah knockout (KO) mutants vis-à-vis wild-type (WT) plants. Plants were grown under short-day conditions on agar plates containing half-strength MS medium with or without 1% sucrose. Notably, the absence of sucrose led to diminished biomass accumulation in both shoot and root tissues of the Atxdh1, Ataln, and Ataah mutants, while no such effect was observed in WT plants. Moreover, the application of sucrose resulted in a reduction of purine degradation metabolite levels, specifically xanthine and allantoin, predominantly within the roots of WT plants. Remarkably, an increase in proteins associated with the purine degradation pathway was observed in WT plants in the presence of sucrose. Lower glyoxylate levels in the roots but not in the shoot of the Atxdh1 mutant in comparison to WT, were observed under sucrose limitation, and improved by sucrose application in root, indicating that purine degradation provided glyoxylate in the root. Furthermore, the deficit of purine-degraded metabolites in the roots of mutants subjected to carbon starvation was partially mitigated through allantoin application. Collectively, these findings signify that under conditions of sucrose limitation and short-day growth, purines are primarily remobilized within the root system to augment the availability of ureides, serving as an additional carbon (as well as nitrogen) source to support plant growth.
Arabidopsis , Carbon starvation , Purine catabolism , Sucrose application , Ureides , Xanthine dehydrogenase
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Biology Department, School of Science and Humanities, Nazarbayev University, Astana, Z05H0P9, Kazakhstan
Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan
The Albert Katz International School for Desert Studies, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus, 8499000, Israel
Jacob Blaustein Center for Scientific Cooperation, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus, 8499000, Israel
The Albert Katz Department of Dryland Biotechnologies, French Associates Institute for Agriculture and Biotechnology of Dryland, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus, 8499000, Israel
Warsaw Univ Life Sci, Inst Biol, Dept Biochem & Microbiol, SGGW, Nowoursynowska 159, Warsaw, PL-02776, Poland
Katif Research Center for Development of Coastal Deserts, Netivot, 87710, Israel
Biology Department
Department of Biotechnology and Microbiology
The Albert Katz International School for Desert Studies
Jacob Blaustein Center for Scientific Cooperation
The Albert Katz Department of Dryland Biotechnologies
Warsaw Univ Life Sci
Katif Research Center for Development of Coastal Deserts
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