Optimal Technological Parameters for Cultivating Trichoderma Consortia Isolated from Northern Kazakhstan Soils
Shumenova N. Nauanova A. Kazangapova N. Yesmurzayeva A. Bekenova S.
1 August 2024International Information and Engineering Technology Association
International Journal of Design and Nature and Ecodynamics
2024#19Issue 41293 - 1300 pp.
This study determined optimal technological parameters (pH of the medium, cultivation temperature, substrate) for the production of the Trichodermin-KZ biofungicide based on consortia of native strains isolated from the soils of Northern Kazakhstan. Three substrates were tested: Czapek-Dox medium, barley seeds, and wheat bran, in liquid and solid forms. Cultural and morphological features of consortia, such as the colour of the aerial and substrate mycelium, the edges of colonies, and the features of fungal sporulation were investigated on agar media. The surface of colonies, the colour of the aerial and substrate mycelium, and the weight of biomass before and after drying were studied on liquid media. Cultivation of the test consortia at different temperatures showed that the optimal temperature is +25℃, at which the colonies reach their maximum diameters: 45.0±0.8; 45.0±0.2; 55.0±0.7; intensive accumulation of biomass, sporulation, and conidia growth. To identify the optimum pH, the fungi were grown at a constant temperature (+25℃), but with variable pH (4.0; 5.0; 6.0; 7.0; 8.0; 9.0); pH 6-7 was found to be optimal for the accumulation of fungal biomass on liquid media, while pH 5-7 was optimal for agar media. Thus, the study established the technological parameters for the production of biofungicide. The materials of this paper are of practical value for producers of organic products.
agricultural crops , antagonistic fungi , biofungicide , Northern Kazakhstan , phytopathogens , technological parameters , Trichoderma , Trichodermin-KZ
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Department of Soil Science and Agrochemistry, S. Seifullin Kazakh Agrotechnical University, Astana, 010011, Kazakhstan
LLP “BIO-KATU”, Astana, 010011, Kazakhstan
Department of Forest Resources, S. Seifullin Kazakh Agrotechnical University, Astana, 010011, Kazakhstan
Faculty of Forestry, Wildlife and Environment, S. Seifullin Kazakh Agrotechnical University, Astana, 010011, Kazakhstan
Department of Biology, Plant Protection and Quarantine, S. Seifullin Kazakh Agrotechnical University, Astana, 010011, Kazakhstan
Department of Soil Science and Agrochemistry
LLP “BIO-KATU”
Department of Forest Resources
Faculty of Forestry
Department of Biology
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