Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. In patients admitted to hospital in Ankara, Turkey

Sarzhanov F. Köster P.C. Dogruman-Al F. Bailo B. Dashti A. Demirel-Kaya F. Carmena D.
April 2021 Cambridge University Press

Parasitology
2021 #148 Issue 5 550 - 561 pp.

This epidemiological study assesses the occurrence of enteric parasites in 4303 patients attended at two public hospitals in Ankara (Turkey) during 2018-2019. Microscopy was used as a screening test. Giardia duodenalis was also identified using a commercial ELISA for the detection of parasite-specific coproantigens. Giardia-positive samples by microscopy/ELISA were confirmed by real-time PCR and characterized using a multilocus genotyping scheme. Blastocystis sp. was genotyped in a sample subset. Blastocystis sp. (11.1%, 95% CI 11.414.8%) and G. duodenalis (1.56%, 95% CI 1.221.96) were the most prevalent pathogens found. Cryptosporidium spp., Entamoeba histolytica and intestinal helminths were only sporadically (<0.5%) found. For G. duodenalis, sequence (n = 30) analyses revealed the presence of sub-assemblages AII (23.3%), discordant AII/AIII (23.3%) and mixed AII + AIII (6.7%) within assemblage A, and BIII (10.0%), BIV (3.3%) and discordant BIII/BIV (23.3%) within assemblage B. Two additional sequences (6.7%) were assigned to the latter assemblage but sub-assemblage information was unknown. No associations between G. duodenalis assemblages/sub-assemblages and sociodemographic and clinical variables could be demonstrated. For Blastocystis sp., sequence (n = 6) analyses identified subtypes ST1, ST2 and ST3 at equal proportions. This is the first molecular characterization of G. duodenalis based on MLG conducted in Turkey to date. Copyright

Blastocystis , Cryptosporidium , Dientamoeba fragilis , Entamoeba histolytica , Giardia duodenalis , intestinal parasites , molecular epidemiology , multilocus genotyping , Turkey

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Department of Medical Microbiology, School of Medicine, Gazi University, Ankara, 06490, Turkey
Faculty of Medicine, Akhmet Yassawi International Kazakh-Turkish University, Turkestan, 161200, Kazakhstan
Parasitology Reference and Research Laboratory, National Centre for Microbiology, Health Institute Carlos Iii, Ctra. Majadahonda-Pozuelo Km 2, Majadahonda Madrid, 28220, Spain
Section of Medical Parasitology, Department of Medical Microbiology, School of Medicine, Gazi University, Ankara, 06490, Turkey
Medical Microbiology Laboratory, Ankara Education and Research Hospital, Health Science University, Ankara, 06230, Turkey

Department of Medical Microbiology
Faculty of Medicine
Parasitology Reference and Research Laboratory
Section of Medical Parasitology
Medical Microbiology Laboratory

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