Phylogenetic analysis of some fungi species in West Kazakhstan based on nuclear ribosomal DNA ITS sequences
Sarsenova A.N. Sevİndİk E. Abievich A.S. Sofyalioğlu E. Esenomanovna D.T. Eken C.
2022Academic Press
Notulae Botanicae Horti Agrobotanici Cluj-Napoca
2022#50Issue 3
In this study, phylogenetic analysis of some fungal species distributed in West Kazakhstan was performed based on ITS sequences. All of the mushroom samples were collected from different regions of West Kazakhstan and brought to the laboratory. Total genomic DNA was extracted using a GeneMark commercial kit. ITS1 and ITS4 primers were used for the amplification of the ITS region in PCR analyses. The resulting DNA sequences were then edited using BioEdit and FinchTV. For phylogenetic analysis used MEGA 6.0 program. As a result of the study, ITS sequences ranged from 532 to 715 nucleotides, while the divergence values of the sequences differed between 0.000 and 0.468. The maximum likelihood tree constructed using ITS sequences consists of two clades. According to the phylogenetic analysis results obtained using other fungal species, ITS results were found to be a good indicator for the differentiation of fungal genera.
Fungi , Its , Phylogenetic analysis , West kazakhstan
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L.N. Gumilyov Eurasian National University, Department of General Biology and Genomics, Satpayev str. 2, Nur-Sultan, Kazakhstan
Aydın Adnan Menderes University, Faculty of Agriculture, Department of Agricultural Biotechnology, South Campus, Cakmar, Aydin, Turkey
Makhambet Utemisov West Kazakhstan University, N. Nazarbayev ave.162, Uralsk, Kazakhstan
L.N. Gumilyov Eurasian National University
Aydın Adnan Menderes University
Makhambet Utemisov West Kazakhstan University
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