Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome
Sandybayev N. Beloussov V. Strochkov V. Solomadin M. Granica J. Yegorov S.
December 2022MDPI
Microorganisms
2022#10Issue 12
The COVID-19 pandemic and heightened perception of the risk of emerging viral infections have boosted the efforts to better understand the virome or complete repertoire of viruses in health and disease, with a focus on infectious respiratory diseases. Next-generation sequencing (NGS) is widely used to study microorganisms, allowing the elucidation of bacteria and viruses inhabiting different body systems and identifying new pathogens. However, NGS studies suffer from a lack of standardization, in particular, due to various methodological approaches and no single format for processing the results. Here, we review the main methodological approaches and key stages for studies of the human virome, with an emphasis on virome changes during acute respiratory viral infection, with applications for clinical diagnostics and epidemiologic analyses.
acute respiratory infections , ARVI , DNA/RNA , metagenomics , NGS , virome , virus
Text of the article Перейти на текст статьи
Kazakhstan-Japan Innovation Center, Kazakh National Agrarian Research University, Almaty, 050010, Kazakhstan
Molecular Genetics Laboratory TreeGene, Almaty, 050009, Kazakhstan
School of Pharmacy, Karaganda Medical University, Karaganda, 100000, Kazakhstan
Michael G. DeGroote Institute for Infectious Disease Research, Faculty of Health Sciences, McMaster University, Hamilton, L8S 4LB, ON, Canada
Kazakhstan-Japan Innovation Center
Molecular Genetics Laboratory TreeGene
School of Pharmacy
Michael G. DeGroote Institute for Infectious Disease Research
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026