Fabrication of Ag nanoparticles coated leonardite basalt ceramic membrane with improved antimicrobial properties for DNA cleavage, E. coli removal and antibiofilm effects

Saleh M. Isik Z. Belibagli P. Arslan H. Gonca S. Özdemir S. Kudaibergenov N. Khataee A. Dizge N.
25 December 2023 Korean Society of Industrial Engineering Chemistry

Journal of Industrial and Engineering Chemistry
2023 #128 532 - 541 pp.

This study aimed to fabricate a novel, low-cost, and environmental-friendly ceramic membrane based on basalt and leonardite powders via press and sintering methods. The fabricated leonardite basalt ceramic membrane (LBCM) was coated with silver nanoparticles (AgNPs); to create an antibacterial surface. The capabilities of the bare and coated membranes were examined. In this context, water permeability reached 554 ± 2.61 and 447 ± 1.21 L/m²hbar for bare LBCM and AgNPs-coated LBCM, respectively. The fabricated membranes indicated 100% Escherichia coli (E. coli) removal efficiency at a transmembrane pressure of 0.5 bar. The solid surface antimicrobial activity of the LBCM and AgNPs-coated LBCM reached 26.38% and 100%, respectively. The LBCM and AgNPs-coated LBCM were analyzed for the in-vitro 2,2- diphenyl-1-picrylhydrazyl (DPPH) antioxidant. Accordingly, AgNPs-coated LBCM exhibited higher antioxidant activities than raw LBCM. The scavenging capacity reached 83.91% with AgNPs-coated LBCM, while only 58.95% was achieved with raw LBCM, indicating that AgNPs-coated LBCM was better than bare LBCM from an antioxidant activities perspective. AgNPs-coated LBCM had a deoxyribonucleic acid (DNA) cleavage activity (single-strand DNA cleavage activity at 50 mg/L and double-strand DNA cleavage activity at 100 and 200 mg/L). In contrast, the raw LBCM did not exhibit DNA cleavage activity at any concentration. AgNPs-coated LBCM showed higher antimicrobial activities (minimum inhibition concentrations (MICs) were 32 mg/L against Enterococcus faecalis (E. faecalis) and 64 mg/L against Staphylococcus aureus (S. aureus), Candida tropicalis (C. tropicalis), and Enterococcus hirae (E. hirae)). The biofilm inhibition of LBCM and AgNPs-coated LBCM powders was tested against S. aureus and Pseudomonas aeruginosa (P. aeruginosa). The maximum S. aureus inhabitations by LBCM and AgNP-coated LBCM were 60.34% and 99.12%, respectively. The inhabitation of P. aeruginosa increased from 52.38% before coating to 96.37% at the end of the coating process. Regarding E.coli microbial cell viability inhibition, LBCM powders and AgNPs-coated LBCM powders were found to inhibit E. coli growth by 68.35% and 100%, respectively.

AgNPs coating , Antimicrobial , Antimicrobial surface , E. coli inhibition , Leonardite basalt composite

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National Agricultural Research Center (NARC), Jenin, Palestine
Mersin University, Department of Environmental Engineering, Mersin, 33343, Turkey
Tarsus University, Department of Energy Systems Engineering, Tarsus, 33400, Turkey
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Mersin, Turkey, Mersin, Yenisehir, TR-33343, Turkey
Food Processing Programme, Technical Science Vocational School, Mersin University, Mersin, Yenisehir, TR-33343, Turkey
Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, al-Farabi 71, Almaty, 050038, Kazakhstan
Department of Environmental Engineering, Gebze Technical University, Gebze, 41400, Turkey
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, 51666–16471, Iran

National Agricultural Research Center (NARC)
Mersin University
Tarsus University
Department of Pharmaceutical Microbiology
Food Processing Programme
Department of Chemistry and Chemical Technology
Department of Environmental Engineering
Research Laboratory of Advanced Water and Wastewater Treatment Processes

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