A new strain Lacticaseibacillus paracasei M12 and perspectives of its use against Erwinia amylovora
Sadanov A. Ismailova E. Shemshura O. Alimzhanova M. Molzhigitova A. Kuldybayev N. Tleubekova D. Daugalieva S. Baimakhanova G. Turlybaeva Z. Yelubayeva A.
August 2024Springer Science and Business Media Deutschland GmbH
Journal of Plant Pathology
2024#106Issue 3925 - 936 pp.
The current investigation was conducted to assess the antagonistic activity of Lacticaseibacillus paracasei M12, a novel strain, against the pathogenic agent Erwinia amylovora E22, which causes fire blight in fruit crops. The E. amylovora E22 strain was isolated from Golden Delicious floral colonization dynamics and specificity of Aureobasidium pullulans strains used to suppress fire blight in apple fruit. The L. paracasei M12 strain was collected from the phyllosphere of the garden cenosis in the Almaty region of Kazakhstan. Identification of both isolates relied on morphological and biochemical tests as well as PCR analysis. Results indicated that the zone of pathogen growth inhibition due to the action of the L. paracasei M12 strain was 41.5 ± 1.5 mm. The maximum inhibitory activity of this antagonist was observed on the 7th day of fermentation. Lactic acid constituted the largest percentage in the cultural broth component composition (43.55%) and exhibited inhibitory effects against E. amylovora E22. Evaluation of various concentrations of the broth in vitro demonstrated that the use of the original broth (100%) resulted in complete inhibition of pathogen growth. A parallel outcome was observed in tests conducted on immature pear fruits, where those treated with 100% of M12 culture broth exhibited complete protection against fire blight infection, while 50% and 20% concentrations showed a slight appearance of the disease. Furthermore, the incorporation of TWEEN® 80 as an adhesive agent enhanced the antagonistic activity of the L. paracasei M12 strain in vitro. Consequently, the L. paracasei strain M12 emerges as a promising candidate for the development of a novel biopesticide targeting fire blight. It was shown that the biological efficacy of L. paracasei M12 against fire blight during double treatment of Aport and Starkrimson fruit trees was 80.3% and 80.7%, respectively.
Apple , Biocontrol , Erwinia amylovora Е22 , Lacticaseibacillus paracasei М12 , Pear
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Scientific Production Center of Microbiology and Virology, Str. Bogenbay Batyr 105, Almaty, 050010, Kazakhstan
Al-Farabi Kazakh National University, Ave. Al-Farabi 71, Almaty, 050040, Kazakhstan
Scientific Production Center of Microbiology and Virology
Al-Farabi Kazakh National University
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