Identification of avian pathogenic E. coli O (somatic) antigens
Sabirovna O.M. Abdulhussein A.H. Abduljabbar M.H. Thabit R. Ali M.L. Dakheel A.H. Alajeeli F. Saleh A.S. Tatarinova G.
April 2023University of Guilan
Caspian Journal of Environmental Sciences
2023#21Issue 2467 - 470 pp.
Infections caused by avian pathogenic Escherichia coli (APEC) seriously threaten poultry production and economic losses. These bacteria have several mechanisms that enable them to colonize various ecosystems. Colibacillosis, especially coli-septicemia form, is the most common bacterial disease in the poultry industry all over the world, with critical economic losses, as well as has public health significance. Tube agglutination test (TAT) is one of the ways for epidemiologic investigation of avian colibacillosis, however, due to the initial dilution rate of 10-1 of antibody, it gives insufficient information. In this paper micro-agglutination test (MAT) method is compared with the TAT method. For this reason, bacteria of E. coli, O2 serogroup, were injected into 14 broiler chickens for 14-day-old. Blood samples were taken 1, 2, and 3 weeks post the injection. Each serum sample was examined with the TAT and MAT methods. The results indicated that the MAT method is more straightforward than the TAT method and can detect lower levels of antibodies to the O (Somatic) antigen of E. coli. The results also indicated that if in the MAT method, the mixtures of antigen and diluted antibodies were incubated at 4 ºC, the titration would have been a good correlation with the TAT method.
Avian colibacillosis , Microagglutination , Serology , Tube agglutination
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Department of Pedagogical Sciences, South Kazakhstan University Named After M. Auezov, Tauke Khan Street 5., Shymkent, Kazakhstan
Department of Pharmaceutics, College of Pharmacy, University of Al-Ameed, Karbala, Iraq
Department of Pharmacy, Al-Noor University College, Nineveh, Iraq
Medical Technical College, Al-Farahidi University, Iraq
Department of Dentistry, Al-Mustaqbal University College, Babylon, Iraq
Medical Technical College, Mazaya University College, Iraq
Medical Technical College, Al-Hadi University College, Baghdad, 10011, Iraq
Medical Technical College, National University of Science and Technology, Dhi Qar, Iraq
Department of Biological Sciences, Institute of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Almaty, Kazakhstan
Department of Pedagogical Sciences
Department of Pharmaceutics
Department of Pharmacy
Medical Technical College
Department of Dentistry
Medical Technical College
Medical Technical College
Medical Technical College
Department of Biological Sciences
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