Obtaining and Use of the Recombinant Bovine Pregnancy-Associated Glycoprotein 1
Ryskeldina A. Iskakova I. Sarin N. Shevtsov A. Syzdykova L. Shustov A. Ramankulov Y. Kuibagarov M.
October 2022ResearchersLinks Ltd
Advances in Animal and Veterinary Sciences
2022#10Issue 102148 - 2159 pp.
A convenient biochemical marker of cow pregnancy is the bovine pregnancy-associated glycoprotein 1 (boPAG1) antigen. The aim of this work was to produce a recombinant boPAG1 antigen and obtain monoclonal antibodies (mAbs) against boPAG1. We have obtained the boPAG1 cDNA and are reporting its nucleotide sequence. Bacterial expression of a portion of the natural gene encoding a mature form of boPAG1 failed. But a fusion protein made up of E. coli thioredoxin and boPAG1 was efficiently expressed in E. coli. Using the recombinant protein as an antigen, mAbs were generated. One mAb reacted with a protein present in extracts from cow placenta. The described results are useful for creating immunological reagents to detect pregnancy in cows, which is of great practical importance.
Bovine pregnancy-associated glycoprotein 1 , Fusion protein , Monoclonal antibodies , Placental antigens , Recombinant antigen
Text of the article Перейти на текст статьи
National Center for Biotechnology, Korgalzhyn hwy 13/5, Nur-Sultan, 010000, Kazakhstan
National Center for Biotechnology
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026