Evaluation of the Method of Periodic Medium Renewal of Bacillus aryabhattai RAF 5 and Analysis of P(3HB) Production
Rysbek A. Jankiewicz U. Pogorzelska-Nowicka E. Wyrwisz J. Abeldenov S. Mirończuk A.M. Richert A.
April 2025Multidisciplinary Digital Publishing Institute (MDPI)
Polymers
2025#17Issue 7
The purpose of this study was to assess the ability of Bacillus aryabhattai RAF 5 to produce P(3HB) under conditions of periodic medium renewal (PMR). The producer was isolated and confirmed using transmission electron microscopy (TEM), which revealed the presence of more than 10 dense P(3HB) granules per cell. The purity of the isolated polymer was evaluated using Fourier transform infrared spectroscopy (FTIR). The maximum concentration of P(3HB) reached 18.70 g/L with biomass accumulation of 21.44 g/L after 120 h of incubation under PMR conditions, which is 6.61 g/L higher than the concentration of P(3HB) obtained with the standard cultivation method. The resulting polymer was later used to create a membrane, which was then tested for permeability to water vapor, oxygen, carbon dioxide, and a biofilm puncture test. The resulting P(3HB)-based membranes have promising barrier properties, indicating their suitability for various industrial applications, including biomedical devices.
Bacillus aryabhattai , periodic medium renewal (PMR) , permeability of membranes , poly(3-hydroxybutyrate) (P(3HB)) , sustainability
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LLP “National Center for Biotechnology”, 13/5, Kurgalzhynskoye Road, Astana, 010000, Kazakhstan
Department of Biochemistry and Microbiology, Faculty of Agriculture and Biology, Warsaw, University of Life Sciences—SGGW, Warsaw, 02-776, Poland
Department of Technique and Food Product Development, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences—SGGW, Warsaw, 02-776, Poland
Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Wroclaw, 51-630, Poland
Institute of Biology, Laboratory for Biosustainability, Wroclaw University of Environmental and Life Sciences, Wroclaw, 51-630, Poland
Department of Genetics, Faculty of Biology and Veterinary Science, Nicolaus Copernicus University, Gagarina 11, Torun, Torun, 87-100, Poland
LLP “National Center for Biotechnology”
Department of Biochemistry and Microbiology
Department of Technique and Food Product Development
Department of Biotechnology and Food Microbiology
Institute of Biology
Department of Genetics
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