Comparative Characterization and Identification of Poly-3-hydroxybutyrate Producing Bacteria with Subsequent Optimization of Polymer Yield

Rysbek A. Ramankulov Y. Kurmanbayev A. Richert A. Abeldenov S.
January-2 2022 MDPI

Polymers
2022 #14 Issue 2

In this work, the strains Bacillus megaterium RAZ 3, Azotobacter chrocococcum Az 3, Bacillus araybhattay RA 5 were used as an effective producer of poly-3-hydroxybutyrate P(3HB). The purpose of the study was to isolate and obtain an effective producer of P(3HB) isolated from regional chestnut soils of northern Kazakhstan. This study demonstrates the possibility of combining the protective system of cells to physical stress as a way to optimize the synthesis of PHA by strains. Molecular identification of strains and amplification of the phbC gene, transmission electron microscope (TEM), extracted and dried PHB were subjected to Fourier infrared transmission spectroscopy (FTIR). The melting point of the isolated P(3HB) was determined. The optimal concentration of bean broth for the synthesis of P(3HB) for the modified type of Bacillus megaterium RAZ 3 was 20 g/L, at which the dry weight of cells was 25.7 g/L⁻¹ and P(3HB) yield of 13.83 g/L⁻¹, while the percentage yield of P(3HB) was 53.75%. The FTIR spectra of the extracted polymer showed noticeable peaks at long wavelengths. Based on a proof of concept, this study demonstrates encouraging results.

Bean broth , Biopolymers , Molecular characterization , P(3HB) , TEM , Ultraviolet irradiation

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National Center for Biotechnology, 13/5 Kurgalzhynskoye Road, Nur-Sultan, 010000, Kazakhstan
Department of General Biology and Genomics, L.N. Gumilyov Eurasian National University, Kazhymukan 13 St., Nur-Sultan, 010000, Kazakhstan
School of Science and Humanities, Nazarbayev University, Qabanbay Batyr Ave 53, Nur-Sultan, 010000, Kazakhstan
Department of Genetics Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, Torun, 87-100, Poland

National Center for Biotechnology
Department of General Biology and Genomics
School of Science and Humanities
Department of Genetics Faculty of Biological and Veterinary Sciences

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