Hepatic gene expression changes in rats internally exposed to radioactive56mno2 particles at low doses
Ruslanova B. Abishev Z. Chaizhunussova N. Shabdarbayeva D. Tokesheva S. Amantayeva G. Kairkhanova Y. Stepanenko V. Hoshi M. Fujimoto N.
September 2021MDPI AG
Current Issues in Molecular Biology
2021#43Issue 2758 - 766 pp.
We have studied the biological effects of the internal exposure to radioactive manganese-56 dioxide (56MnO2), the major radioisotope dust found in soil after atomic bomb explosions. Our previous study of blood chemistry indicated a possible adverse effect of56MnO2 on the liver. In the present study, we further examined the effects on the liver by determining changes in hepatic gene expressions. Male Wistar rats were exposed to56MnO2 particles (three groups with the whole-body doses of 41, 90, and 100 mGy), stable MnO2 particles, or external60Co γ-rays (2 Gy), and were examined together with the non-treated control group on postexposure day 3 and day 61. No histopathological changes were observed in the liver. The mRNA expression of a p53-related gene, the cyclin-dependent kinase inhibitor 1A, increased in56MnO2 as well as in γ-ray irradiated groups on postexposure day 3 and day 61. The expression of a stress-responsive gene, nuclear factor κB, was also increased by56MnO2 and γ-rays on postexposure day 3. However, the expression of cytokine genes (interleukin-6 or chemokine ligand 2) or fibrosis-related TGF-β/Smad genes (Tgfb1, Smad3, or Smad4) was not altered by the exposure. Our data demonstrated that the internal exposure to56MnO2 particles at less than 0.1 Gy significantly affected the short-term gene expressions in the liver in a similar manner with 2 Gy of external γ-irradiation. These changes may be adaptive responses because no changes occurred in cytokine or TGF-β/Smad gene expressions.
Hepatic gene expression , Internal radiation exposure , Radiation-induced liver damages , Radiation-sensitive genes , Residual radiation
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Department of Pathological Anatomy and Forensic Medicine, Semey Medical University, Semey, 071400, Kazakhstan
Department of Public Health, Semey Medical University, Semey, 071400, Kazakhstan
Department of Microbiology, Semey Medical University, Semey, 071400, Kazakhstan
National Medical Research Center of Radiology, Ministry of Health of Russian Federation, Obninsk, 249031, Russian Federation
The Center for Peace, Hiroshima University, Hiroshima, 7300053, Japan
Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, 7340037, Japan
Department of Pathological Anatomy and Forensic Medicine
Department of Public Health
Department of Microbiology
National Medical Research Center of Radiology
The Center for Peace
Research Institute for Radiation Biology and Medicine
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