In vitro α-glycosidase and urease enzyme inhibition profile of some selected medicinal plants of Pakistan
Rauf A. Bawazeer S. Naseer M. Alhumaydhi F.A. Aljohani A.S.M. Habib A. Khan R. Jehan U. Qureshi M.N. Khan M. Farooq U. Kassenov A. Shariati M.A.
2021Taylor and Francis Ltd.
Natural Product Research
2021#35Issue 235434 - 5439 pp.
The current study aims at exploring enzyme inhibition of four species of medicinal herbs, namely Senna bicapsularis, Thevetia peruviana, Nerium oleander and Vinca major. Plant selection was done on the basis of their therapeutic uses by local practitioners. The crude methanolic extracts of these plants were tested for their α-glycosidase and urease enzyme inhibition potential. The observed urease inhibitory potential for the crude extract of S. bicapsularis, T. peruviana and N. oleander were 8.3 ± 0.33 μg, 6.98 ± 0.98 μg and 9.56 ± 1.43 μg, respectively while the V. major did not show any inhibition. In addition, the IC50 value for Thiourea was 22.3 ± 1.14 μg. The crude extracts of S. bicapsularis, T. peruviana, N. oleander, V. major were shown to inhibit α-glycosidase activity with an IC50 value of 630.3 ± 0.03 μg, 700.7 ± 2.43 μg, 430.4 ± 3.97 μg, and the standard (acarbose) 880 ± 1.03 μM, respectively. Based on the TLC profile, the extract of S. bicapsularis was subjected to column chromatography and the major component named rhein (1) was identified. Compound 1 exhibited excellent urease and α-glycosidase inhibitory activity with an IC50 value of 7.4 ± 0.32 and 622.3 ± 1.03 μM, respectively.
Nerium oleander , phytochemicals , Senna bicapsularis , Thevetia peruviana , urease activity , Vinca major , α-glycosidase
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Department of Chemistry, University of Swabi, Swabi, Pakistan
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia
Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia
H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
Department of Chemistry, COMSATS University Islamabad, Abbottabad, Pakistan
Kazakhstan Kazakh Research Institute of Processing and Food Industry LLP, Shakarim State University of Semey, Semey, Kazakhstan
Laboratory of Biocontrol and Antimicrobial Resistance, Orel State University Named After I.S. Turgenev, Orel, Russian Federation
Department of Chemistry
Department of Pharmaceutical Chemistry
Department of Medical Laboratories
Department of Veterinary Medicine
H.E.J. Research Institute of Chemistry
Department of Chemistry
Kazakhstan Kazakh Research Institute of Processing and Food Industry LLP
Laboratory of Biocontrol and Antimicrobial Resistance
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