Triple-cation CoPrFe-layered double hydroxide: Harnessing peroxidase-mimicking activity, antibacterial, and anticancer potentials in a single system
Rashtbari S. Dehghan G. Khataee A. Khataee S. Arefi-Oskoui S. Kudaibergenov N. Alikeyeva A.
October 2025Elsevier Ltd
Journal of Environmental Chemical Engineering
2025#13Issue 5
Nanomaterial-based therapies are promising tools to combat bacterial infections and efficiently target cancer cells. In the present study, we synthesized praseodymium-doped CoFe-layered double hydroxide (CoPrFe ternary LDH) and characterized it using different advanced methods. The produced LDH demonstrated strong peroxidase-like performance, speeding up the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 to yield a bluish-colored product. The experimental conditions, pH and temperature, were optimized, and steady-state kinetic parameters were calculated. The obtained Km values were lower than those of natural peroxidases, suggesting the superior catalytic potential of CoPrFe-LDH. Furthermore, the bactericidal performance of the synthesized CoPrFe-LDH was examined against two model bacterial strains. The minimum inhibitory concentration values were obtained and the results demonstrated the higher sensitivity of S. aureus towards CoPrFe-LDH. The FE-SEM images and fluorescence microscopy results indicated that the cell membrane damage could be considered the main antibacterial mechanism of CoPrFe-LDH. Furthermore, the anticancer activity of CoPrFe-LDH was checked, and it was shown that the LDH induces apoptosis in the breast cancer cell line (MDA-MB-231), and the half-maximal inhibitory concentration value was estimated to be 38.17 μg/mL. This work may offer new approaches to creating unique LDH-derived nanoenzymes for use in biotechnology and medicine.
Antibacterial , CoPrFe-LDH , Cytotoxicity , Layered double hydroxide , Nanozyme , Peroxidase mimicking activity
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Laboratory of Biochemistry and Molecular Biology, Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, 51666-16471, Iran
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, 51666-16471, Iran
Department of Chemical Engineering, Istanbul Technical University, Istanbul, 34469, Turkey
Center of Physical Chemical Methods of Research and Analysis, Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, 050038, Kazakhstan
Laboratory of Biochemistry and Molecular Biology
Research Laboratory of Advanced Water and Wastewater Treatment Processes
Department of Chemical Engineering
Center of Physical Chemical Methods of Research and Analysis
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