Functionalized optical fiber ball-shaped biosensor for label-free, low-limit detection of IL-8 protein
Rashidova G. Tilegen M. Pham T.T. Bekmurzayeva A. Tosi D.
1 January 2024Optica Publishing Group (formerly OSA)
Biomedical Optics Express
2024#15Issue 1185 - 198 pp.
Detection of biomarkers for tracking disease progression is becoming increasingly important in biomedicine. Using saliva as a diagnostic sample appears to be a safe, cost-effective, and non-invasive approach. Salivary interleukin-8 levels demonstrate specific changes associated with diseases such as obstructive pulmonary disease, squamous cell carcinoma, oral cancer, and breast cancer. Traditional protein detection methods, such as enzyme-linked immunosorbent assay (ELISA), mass spectrometry, and Western blot are often expensive, complex, and time-consuming. In this study, an optical fiber-based biosensor was developed to detect salivary IL-8 protein in a label-free manner. The biosensor was able to achieve an ultra-low limit detection of 0.91 fM. Moreover, the tested concentration range was wide: from 273 aM to 59 fM. As a proof-of-concept for detecting the protein in real clinical samples, the detection was carried out in artificial saliva. It was possible to achieve high sensitivity for the target protein and minimal signal alterations for the control proteins.
Text of the article Перейти на текст статьи
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, 010000, Kazakhstan
National Laboratory Astana, Nazarbayev University, Astana, 010000, Kazakhstan
School of Engineering and Digital Sciences, Nazarbayev University, Astana, 010000, Kazakhstan
Department of Biology
National Laboratory Astana
School of Engineering and Digital Sciences
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026