Molecular Genetic Identificatin of Plants of the Genus Artemisia L. Growing in Southern Regions of Kazakhstan
Rakhimberdiyeva Z. Kaliyeva A. Aksoy A. Kassymbayev B. Medeuova G.
September 2022Hashemite University
Jordan Journal of Biological Sciences
2022#15Issue 3501 - 509 pp.
The purpose of this research study is to carry out a molecular genetic identification of the plant species of the genus Artemisia L. growing in Southern Kazakhstan. Such methods as barcoding, PCR analysis, sequencing and electrophoresis were involved. In the course of DNA barcoding, universal plastidial markers for plants were used: rbcL, matK and trnHpsbA. Three samples of Artemisia L. plants were studied for further determination of genetic relationship between species, and identification thereof. Molecular genetic passportization was done based on the obtained results. Molecular genetic analysis enabled identification of the species-level for Artemisia L. samples, and selection of the most promising plants. It was demonstrated that all samples belong to the same species, namely Artemisia annua L. The longest genetic distance between contrast samples is 0.9%. The advised methodology may serve as a model for genetic passportization of resource plant species, as well as an approach to assessing the state of gene pools in population.
Artemisia l , Dna markers , Genetic analysis , Genetic passportization , Molecular markers
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Kazakh National Women’s Teacher Training University, 99 Aiteke bi St., Almaty, Kazakhstan
Akdeniz Üniversitesidisabled, Konyaalti, Antalya, 07070, Turkey
Kazakh National Agrarian University, 8 Albay St., Almaty, 050010, Kazakhstan
Kazakh National Women’s Teacher Training University
Akdeniz Üniversitesidisabled
Kazakh National Agrarian University
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