Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors
Rakhimbekova A. Kudaibergenov B. Seitkamal K. Bellone A. Dauletova A. Sypabekova M. Olivero M. Perrone G. Radaelli A. Zanotto C. De Giuli Morghen C. Vangelista L. Tosi D.
December 2023Nature Research
Scientific Reports
2023#13Issue 1
In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclonal anti-L1R antibody targeting the L1R protein, and the polyclonal rabbit serum antibodies targeting the whole vaccinia virus (VV) pathogen. Experimental measurements were carried out to detect VV in concentrations from 103 to 108 plaque-forming units (PFU), with a limit of detection of around 1.7–4.3 × 103 PFU and a log-quadratic pattern, with a response up to 5 × 10−4 RIU (refractive index units). The specificity was assessed against herpes simplex virus, used as a non-specific control, with the best results obtained with anti-L1R monoclonal antibodies, and through the detection of vaccinia virus/herpes simplex-1 combination. The obtained results provide a real-time viral recognition with a label-free sensing platform, having rapid response and ease of manufacturing, and paving the road to the seamless detection of poxviruses affecting different human and animal species using optical fibers.
Text of the article Перейти на текст статьи
Department of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev University, Astana, 010000, Kazakhstan
Department of Biomedical Sciences, School of Medicine, Nazarbayev University, Astana, 010000, Kazakhstan
Department of Electronics and Telecommunications, Politecnico Di Torino, Turin, Italy
Department of Electrical & Computer Engineering, Baylor University, Waco, TX, United States
Department of Medical Biotechnologies and Translational Medicine, Laboratory of Molecular Virology and Recombinant Vaccine Development, University of Milan, Via Vanvitelli 32, Milan, Italy
Catholic University “Our Lady of Good Counsel”, Rr. Dritan Hoxha, Tirana, Albania
Department of Molecular Medicine, University of Pavia, Pavia, 27100, Italy
Laboratory of Biosensors and Bioinstruments, National Laboratory Astana, Astana, 010000, Kazakhstan
Department of Electrical and Computer Engineering
Department of Biomedical Sciences
Department of Electronics and Telecommunications
Department of Electrical & Computer Engineering
Department of Medical Biotechnologies and Translational Medicine
Catholic University “Our Lady of Good Counsel”
Department of Molecular Medicine
Laboratory of Biosensors and Bioinstruments
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026