Serum and Urinary Proteomic Signatures Revealing Redox and Metabolic Dysregulation in Acute Achilles Tendon Rupture
Qianman B. Jiasharete T. Wupuer A. Tuerxun A. Jialihasi A. Mamately A. Yeerbo N. Shawutali N. Badalihan A. Aisaiding A. Redati D. Wuerliebieke J. Aizezi A. Bahesutihan Y. Zhao B. Ainiwaer N. Jielile J.
2025Bentham Science Publishers
Protein and Peptide Letters
2025#32Issue 6437 - 450 pp.
Introduction: The etiology of acute Achilles tendon rupture (ATR) remains unclear. This study conducted a comprehensive case-control study of the proteome profile to gain insights into the potential pathogenesis of acute ATR and identify novel biomarkers. Methods: Serum (iTRAQ) and urine (label-free proteomics) from 15 acute ATR patients and 15 healthy controls were analyzed. Significant differential expression was defined as ≥1.2-fold (serum) or ≥2-fold (urine) change with p < 0.05. Bioinformatics analyses (GO, KEGG, PPI) were performed. Results: 44 serum and 198 urine proteins were differentially expressed. Enriched pathways included immune response, metabolism, immune response, and redox regulation. protein-protein interaction analysis of the differentially expressed proteins (P < 0.05) highlighted abnormalities in major protein-protein interaction hubs, specifically pyruvate kinase (PKM), peroxiredoxin-1 (PRDX1), phosphoglycerate kinase 1 (PKG1), profilin-1, and apolipoprotein A-IV, observed in the serum and urine samples of acute ATR patients. Discussion: Metabolic dysregulation may affect tendon structure/strength; redox imbalance could promote degeneration. Immune-related proteins may reflect injury responses. Glycolytic enzymes (PKM, PGK1) suggest disrupted energy metabolism. Conclusion: Proteomic abnormalities in metabolism, immune, and redox pathways, along with key proteins (PKM, PRDX1, PGK1), may contribute to ATR pathogenesis, offering potential biomarkers warranting further validation.
Achilles tendon rupture , biomarkers , ITRAQ , label-free proteomics , proteome profile , system biology
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Department of Osteopathy and Orthopedics (Ankle) Surgery, The Sixth Teaching Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, China
Department of Clinical Medicine, Capital Medical University Class 2024, Five-year Clinical Medicine Class 3, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing, 100069, China
Clinical Medical Research Center, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang, Urumqi, China
Department of Orthopedics, Almaty Medical Center HAK, Almaty, 050063, Kazakhstan
Department of Orthopedic Centre, The Fifth Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, China
Department of Geriatric Joint Surgery of Orthopedics, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Department of Pediatric Surgery, Childrens Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Department of Orthopedics, Xinjiang Urumqi International (Eye and Otorhinolaryngological) Hospital, No.50 Lianhu Road, Toutunhe District Urumqi, Xinjiang, Urumqi, 830000, China
Department of Hand and Foot Microsurgery, Childrens Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Uygur Autonomous Region, Urumqi, China
Department of Gastrointestinal Surgery, The Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi, China
Department of Orthopedics, People’s Hospital of Altay Region, Xinjiang, Altay, 836500, China
Trauma Orthopedics Department, Prefecture Second People’s Hospital of Xinjiang Uygur Autonomous Region, Xinjiang, Kashgar, Kashgar, 844000, China
Department of Orthopedics Surgery, Xinjiang 474 Hospital, Urumqi, Xinjiang Uygur Autonomous Region, Urumqi, 474, China
Department of Osteopathy and Orthopedics (Ankle) Surgery
Department of Clinical Medicine
Clinical Medical Research Center
Department of Orthopedics
Department of Orthopedic Centre
Department of Geriatric Joint Surgery of Orthopedics
Department of Pediatric Surgery
Department of Orthopedics
Department of Hand and Foot Microsurgery
Department of Gastrointestinal Surgery
Department of Orthopedics
Trauma Orthopedics Department
Department of Orthopedics Surgery
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