iTRAQ-Based Proteomic Analysis of Spontaneous Achilles Tendon Rupture
Qianman B. Jiasharete T. Badalihan A. Mamately A. Yeerbo N. Bahesutihan Y. Wupuer A. Aisaiding A. Wuerliebieke J. Jialihasi A. Li P. Jielile J.
3 January 2025American Chemical Society
Journal of Proteome Research
2025#24Issue 165 - 76 pp.
Spontaneous Achilles tendon rupture (SATR) predominantly affects middle-aged and elderly individuals with chronic injuries. However, the exact cause and mechanism of SATR remain elusive, and potential therapeutic intervention or prevention is still insufficient. The present study aimed to uncover the key pathological molecules by using iTRAQ proteomics. The results identified 2432 candidate proteins in SATR patients using iTRAQ proteomic analysis. A total of 307 differentially expressed proteins (DEPs) were identified and linked to 211 KEGG signaling pathways including Coronavirus disease (COVID-19), focal adhesion, and ribosomes. GO enrichment analysis highlighted significant enrichment in processes such as biological adhesion, ossification, lipid (APOA4) processes, and extracellular matrix (ECM) organization (collagen). PPI network analysis identified hub genes such as serum albumin (ALB), fibronectin (FN1), and actin cytoplasmic 1. The WB analysis confirmed that FN1 and the receptor for activated C kinase (RACK1) were downregulated in the SATR tendon. Immunohistochemical staining revealed that collagen I and III were suppressed, while collagen II and APOA4 expression were higher in the SATR pathological tissue (P < 0.05). However, the primary cultured tenocytes (PCTs) from SATR patients showed enhanced proliferation and, consistent with tissue staining, reduced collagen I and III and increased collagen II. Our findings reveal vital targets and pathways in SATR’s etiological progression, offering a new perspective on the diagnosis, treatment, and prognosis of this complex disorder.
Etiology , iTRAQ , Proteomics , Spontaneous Achilles tendon rupture , Systems biology
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Department of Osteopathy and Orthopedics (Ankle) Surgery, The Sixth Teaching Hospital of Xinjiang Medical University, No. 39 Wuxing South Road, Xinjiang Uygur Autonomous Region, Urumqi, 830001, China
Department of Clinical Medicine, Capital Medical University Class of 2024, Five-Year Clinical Medicine Class 3, No. 10, Xitoutiao, You’anmenwai, Fengtai District, Beijing, 100069, China
Department of Orthopedics, Xinjiang Urumqi International and Otorhinolaryngological Hospital, No. 50 Lianhu Road, Toutunhe District Urumqi, Xinjiang, Urumqi, 830000, China
Department of Orthopedic Centre, The Fifth Affiliated Hospital of Xinjiang Medical University, No. 118 West Henan Road, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China
Department of Geriatric Joint Surgery of Orthopedics, People’s Hospital of Xinjiang Uygur Autonomous Region, No. 91 Tianchi Road, Urumqi, 830001, China
Department of Orthopedics Surgery, Xinjiang 474 Hospital, No. 754, Beijing Middle Road, Beijing North Road, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China
Department of Hand and Foot Microsurgery, Xinjiang Uygur Autonomous Region Children’s Hospital, No. 393 Altay Road, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China
Department of Orthopedics, People’s Hospital of Altay Region, No. 31, Park Road, Xinjiang, Altay, 836500, China
Department of Orthopedics, Almaty Medical Center HAK, Kazakhstan, No. 11a Otegenbater Road, Almaty, 050063, Kazakhstan
Department of Microrepair and Reconstruction of Orthopedics Centre, The First Teaching Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830054, China
Department of Osteopathy and Orthopedics (Ankle) Surgery
Department of Clinical Medicine
Department of Orthopedics
Department of Orthopedic Centre
Department of Geriatric Joint Surgery of Orthopedics
Department of Orthopedics Surgery
Department of Hand and Foot Microsurgery
Department of Orthopedics
Department of Orthopedics
Department of Microrepair and Reconstruction of Orthopedics Centre
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