Streptomycin-modified magnetic beads enable robust DNA extraction across diverse lysis conditions
Qi Y. Zheng J. Xie Y. Amin A. Qiao B. Shi C. Li Y. Ma C.
July 2025Academic Press Inc.
Analytical Biochemistry
2025#702
Here, we describe the applicability of a variety of functionalized magnetic beads (MBs) for DNA extraction under different lysis conditions. Various MBs, including hydroxyl, carboxylic, amino, chitosan-modified, and streptomycin-modified MBs (MBs-STR), were compared based on their extracted DNA quantity and quality. The results presented that the MBs exhibited different DNA adsorption capacities under specific conditions. Notably, MBs-STR maintained stable DNA adsorption capacity upon different conditions through a non-intercalative binding mechanism between STR and DNA minor groove. This discovery advances antibiotic-based DNA extraction strategies, offering a flexible, efficient alternative for biomedical applications while enriching the toolkit for nucleic acid isolation.
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College of Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, 010000, Kazakhstan
Basic Medical Sciences, College of Medicine, University of Sharjah, Sharjah, 27272, United Arab Emirates
Animal Husbandry and Veterinary Center Station of Yangting Town, Huancui District, Weihai, 264204, China
College of Life Sciences, Qingdao University, Qingdao, 266071, China
College of Biological Engineering
Department of Biology
Basic Medical Sciences
Animal Husbandry and Veterinary Center Station of Yangting Town
College of Life Sciences
10 лет помогаем публиковать статьи Международный издатель
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