Luminescence Spectral Properties of New Benzothiazole Polymethine Dye
Pligin E.I. Lavysh A.V. Lugovskii A.A. Voropay E.S. Kopishev É.E. Maskevich A.A.
January 2023Springer
Journal of Applied Spectroscopy
2023#89Issue 61021 - 1028 pp.
The absorption and fluorescence spectra of a new styryl derivative of thioflavin T 2-{(1E,3E)-4-[4-(dimethylamino)-2,6-dimethylphenyl]buta-1,3-dien-1-yl}-3-ethyl-1,3-benzothiazol-3-ium tosylate (Th-C23) in solvents with different polarity and viscosity and also incorporated in the structure of amyloid fibrils and bovine serum albumin were investigated. A characteristic feature of the dye is an extremely low quantum yield of fluorescence in low-viscosity solvents (10–4 in water) which, however, increases significantly in viscous solutions and when it is incorporated in the structure of proteins or amyloid fibrils. In the latter case the quantum yield increases by 8∙103 times. On the basis of the experimental studies and quantum chemical calculations it was shown that Th-C23 exhibits the properties of a molecular rotor. The increase of the fluorescence quantum yield in viscous solutions and in the biopolymers results from limitation of the torsional rotation of the molecular fragments, leading to fluorescence quenching. The long-wavelength location of the absorption spectrum and the fluorescence spectrum of the new dye in the red region of the spectrum (520 and 600 nm) makes it possible to use it as a fluorescent marker that is sensitive to the viscosity (hardness) of the microenvironment not only in vitro but also in vivo.
Amyloid fibrils , Fluorescent molecular rotors , Styryl derivatives of thioflavin T
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Yanka Kupala Grodno State University, Grodno, Belarus
Belarus State University, Minsk, Belarus
L. N. Gumilev, Eurasian National University, Astana, Kazakhstan
Yanka Kupala Grodno State University
Belarus State University
L. N. Gumilev
10 лет помогаем публиковать статьи Международный издатель
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