Isolation of Lactobacillus Strains with Probiotic Activity to be used as a Fish Feed Supplement
Paritova A.Y. Balji Y.A. Murzakayeva G.K. Aytkozhina B.Z. Zhanabayev A.A. Kuzembekova G.B. Ashimova K.K. Abulgazimova G.A. Kuanchaleyev Z.B. Askambayeva D. Mayer Y.
May-June 2025Unique Scientific Publishers
International Journal of Veterinary Science
2025#14Issue 3527 - 534 pp.
The paper reports the results of studies on the isolation of Lactobacillus strains with probiotic properties for use as a fish feed supplement. For this purpose, a pure culture of Lactobacillus strains with probiotic activity was isolated from the intestines of fish, and molecular genetic identification of the isolated Lactobacilli was carried out by 16S RNA sequencing following the Sanger method. The amplicons of 20 bacterial samples with a fragment length of 790bp were obtained. The results of nucleotide sequence decoding are as follows: two nucleotide sequences were not identified, and fish pathogens causing intestinal disorder belonging to three species (Kurthia gibsonii, Kurthia zopfii, and Lactococcus garvieae) were identified from eight sequences. The remaining 10 strains were identified as Lactobacillus belonging to two species: Leuconostoc mesenteroides and Lactococcus lactis. A phylogenetic tree was constructed using the nucleotide sequences. Catalase activity determination results confirm that the L. mesenteroides and L. lactis strains do not possess catalase activity. In contrast, air bubbles were formed when K. gibsonii strain was used, indicating catalase activity. These findings highlight the potential of L. mesenteroides and L. lactis as probiotic strains for developing functional fish feed supplements.
Fish feed supplement , Lactobacillus Strains , Nucleotide sequences , Probiotic activity
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S. Seifullin Kazakh Agro Technical Research University, Astana, Kazakhstan
S. Seifullin Kazakh Agro Technical Research University
10 лет помогаем публиковать статьи Международный издатель
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