Cytological Analysis of Upper Respiratory Tract Epithelial Cells in Chrysotile Asbestos Factory Workers
Otarov Y. Zharylkassyn Z. Shaibek A. Mukasheva M. Sabirov Z. Alexeyev A. Izdenov A. Ismailov C. Tilemissov M. Dossybayeva G. Zhaketayeva N. Shaikhattarova U.
March 2025Multidisciplinary Digital Publishing Institute (MDPI)
Life
2025#15Issue 3
Objective: This study aims to assess the impact of prolonged occupational exposure to chrysotile asbestos on the epithelial cells of the upper respiratory tract and the levels of surfactant protein D (SP-D) in female workers. Methods: Buccal epithelial cell samples were collected from 40 workers at JSC “Kostanay Minerals”, fixed using the May–Grünwald method, and stained with the Romanowsky–Giemsa technique. SP-D levels were measured using an enzyme-linked immunosorbent assay (ELISA). Results: Workers exposed to asbestos dust exhibited a significant increase in cytological abnormalities and higher SP-D levels compared to the control group. Conclusion: Prolonged exposure to chrysotile-containing dust leads to degenerative changes in upper respiratory tract epithelial cells, characterized by cytological and cytogenetic abnormalities, alongside elevated SP-D levels, highlighting the need for preventive health measures.
buccal epithelium , chrysotile , micronuclei , surfactant protein D
Text of the article Перейти на текст статьи
NC JSC National Centre Occupational Health and Diseases, 15 Mustafin Street, Karaganda, 100008, Kazakhstan
School of Public Health, JSC Karaganda Medical University, 40 Gogol Street, NC, Karaganda, 100008, Kazakhstan
Department of Zoology, NC JSC Karagandy University named after E. A. Buketov, 28 University Street, Karaganda, 100026, Kazakhstan
Department of Physiology, NC JSC Karagandy University named after E. A. Buketov, 28 University Street, Karaganda, 100026, Kazakhstan
Institute of Life Sciences, NC JSC Karaganda Medical University, 40 Gogol Street, Karaganda, 100008, Kazakhstan
Department of Medical Education, Ministry of Healthcare of the Republic of Kazakhstan, Mangilik El str., 8, Astana, 010000, Kazakhstan
Department of General Practitioner-2, South Kazakhstan Pharmaceutical Academy, Al-Farabi Square 1, Shymkent, 160019, Kazakhstan
Nursing Education School, JSC Karaganda Medical University, 40 Gogol Street, NC, Karaganda, 100008, Kazakhstan
Department of Public Health and Scientific Research, Khoja Akhmet Yassawi International Kazakh-Turkish University, 73 Almaty Street, Turkestan 161200, Kazakhstan
NC JSC National Centre Occupational Health and Diseases
School of Public Health
Department of Zoology
Department of Physiology
Institute of Life Sciences
Department of Medical Education
Department of General Practitioner-2
Nursing Education School
Department of Public Health and Scientific Research
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026