Phytochemical Profile and Biological Activity of the Ethanol Extracts from the Aerial Parts of Adonis tianschanica (Adolf.) Lipsch. Growing in Kazakhstan
Orynbekova S. Kukula-Koch W. Sakipova Z. Alsharif B. Rafferty B. Nurgozhin T. Allambergenova Z. Dreher P. Głowniak K. Boylan F.
December 2024Multidisciplinary Digital Publishing Institute (MDPI)
Molecules
2024#29Issue 23
Adonis tianschanica is a lesser-known plant species belonging to the genus Adonis that grows in Kazakhstan. The aim of this study was to characterize the composition of the ethanolic, water, and hydroethanolic extracts from the aerial parts of A. tianschanica by HPLC-ESI-QTOF-MS/MS to isolate the major compound isoquercitrin by HSCCC (High-Speed Counter-Current Chromatography) and to determine the cytotoxicity and anti-inflammatory potential of the extracts produced with this plant. Fingerprinting of the analyzed extracts showed the presence of a multitude of metabolites comprising polyphenols, organic acids, and coumarins, and only trace quantities of cardiac glycosides in the analyzed samples. Flavonoids were certainly the best-represented group, with kaempferol, quercetin, and their derivatives as the major components of the extracts. Key findings in this paper were that the ethanol: water (50:50 v/v) extract of A. tianschanica and its major compound isoquercitrin were able to reduce the production of NO induced by LPS, in addition to demonstrating anti-inflammatory effects by reducing cytokines such as IL-6, TNF-α, and IL-1β.
Adonis tianschanica(Adolf.) Lipsch , anti-inflammatory activity , cytotoxic effects , flora of Kazakhstan , HPLC-MS fingerprinting , HSCCC , isoquercitrin , NO production
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School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Almaty, 050012, Kazakhstan
Department of Pharmacognosy with Medicinal Plant Garden, Medical University of Lublin, 1 Chod’zki Street, Lublin, 20-093, Poland
School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Dublin, D02 PN40, Ireland
Faculty of Pharmacy, Umm Al-Qura University, Makkah, 21955, Saudi Arabia
School of General Medicine, Asfendiyarov Kazakh National Medical University, Almaty, 050012, Kazakhstan
Department of Public Health, Faculty of Medicine, Medical University of Lublin, Lublin, 20-093, Poland
Department of Cosmetology, The University of Information Technology and Management in Rzeszow, Sucharskiego 2, Rzeszow, 35-225, Poland
School of Pharmacy
Department of Pharmacognosy with Medicinal Plant Garden
School of Pharmacy and Pharmaceutical Sciences
Faculty of Pharmacy
School of General Medicine
Department of Public Health
Department of Cosmetology
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