Intensification of bioremediation of oil-contaminated soils using a natural consortium of microorganisms
Oralbayeva K.B. Yernazarova A.K. Kundybayev M.E. Seitkulov S.A. Kistaubayeva A.S.
2021Transneft Media, LLC
Science and Technologies: Oil and Oil Products Pipeline Transportation
2021#11Issue 5582 - 589 pp.
Currently biological methods of cleaning oily soils significantly developed as the most accessible and effective. In their basis – the use of hydrocarbon-oxidizing microorganisms (HOM) metabolic potential. At the same time, as practice shows, existing preparations containing HOM are not effective enough in extreme soil-climatic conditions, in this connection the search for indigenous strains of oil-oxidizing bacteria and the development of new biological preparations are of current interest. The aim of this work is to study the effectiveness of HOM strains for restoring the biological activity of oil-contaminated soil. A study of the microflora of unpolluted and oil-contaminated soil taken from the experimental site of the “Shymkent” oil pumping station of “KazTransOil” JSC was carried out: the total number of microorganisms was 210 ·106 cells/g and 130·106 cells/g, respectively. From 16 НOM strains isolated from oil-contaminated soil, 3 bacterial strains were selected to create an association and use as a biological product-oil destructor – P12, P16, A8. Based on the results of genotyping, strains P12 and P16 correspond to the species Pseudomonas aeruginosa, strain A8 belongs to the species Alcaligenes denitrificans. The associations of Pseudomonas sp. P16, Pseudomonas sp. P12 and Alcaligenes sp. A8 showed better growth in comparison with monocultures on a nutrient mediumwith hydrocarbons. Bioremediation of artificially oil polluted site process, which carried out for 120 days using a biological product created on the basis of the association of strains P12, P16 and A8, decreased the content of hydrocarbons 3.4 times, and with additional application of mineral fertilizers (ammophos) – 4.6 times. Thus, proved the expediency of using HOM strains P12, P16, A8 for the purpose of bioremediation of oil-contaminated soils. The results of the study can be used to improve economical and environmental efficiency of work when carrying out measures to eliminate the consequences of oil spills on the ground.
Bacteria-destructors , Biocenoses , Bioremediation , Microorganisms-destructors , Oil-contaminated soil , Strainsdestructors
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Research and Development Centre of KazTransOil, JSC, 154, Zhibek Zholy Str., Almaty, 050000, Kazakhstan
Al-Farabi Kazakh National University, 71 Al-Farabi Prospect, Almaty, 050040, Kazakhstan
KazTransOil JSC, Shymkent Oil Pipeline Management Department, 68B Zhibek Zholy Prospect, Shymkent, 160016, Kazakhstan
Research and Development Centre of KazTransOil
Al-Farabi Kazakh National University
KazTransOil JSC
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