Phenolic fingerprinting, bioactivity, and chemometric classification of Tamarix and Agrimonia monofloral honeys from Kazakhstan
Ongalbek D. Tokul-Ölmez Ö. Şahin B. Aydogmus-Ozturk F. Küçükaydın S. Sıcak Y. Yeskaliyeva B. Ozturk M.
15 May 2026Elsevier Ltd
Food Chemistry
2026#511
Kazakhstan has unique nectar sources like Tamarix and Agrimonia, but their monofloral honeys chemical and bioactive properties are still underexplored. This study thoroughly characterized Tamarix ( n = 16) and Agrimonia ( n = 10) honeys from three regions of Kazakhstan, focusing on their phenolic content, antioxidant, and enzyme-inhibitory activities, using chemometric techniques. The botanical origin of the honeys, meeting international quality standards, was confirmed through melissopalynological analysis. Phenolic profiling identified twenty compounds, with gallic acid (793.6 ± 63.5–1024.7 ± 76.4 mg/kg), p -hydroxybenzoic acid (0.96 ± 0.46–42.67 ± 24.44 mg/kg), p -hydroxyphenylacetic acid (0.96 ± 0.52–213.3 ± 102.6 mg/kg), coumarin (9.10 ± 4.53–57.48 ± 8.19 mg/kg), ferulic acid (19.70 ± 1.84–47.93 ± 17.28 mg/kg), and caffeic acid (1.35 ± 0.78–10.88 ± 3.18 mg/kg) as major constituents depending on mg/kg geographical origin. Antioxidant activity was assessed using five complementary assays, while enzyme inhibitory activities were tested against acetylcholinesterase, butyrylcholinesterase, urease, and tyrosinase. Tamarix honeys generally showed stronger antioxidant and anticholinesterase activities, whereas Agrimonia honeys demonstrated significant radical scavenging potential. PCA and HCA successfully classified honeys by botanical and geographical origin, confirming that phenolic fingerprints serve as reliable chemotaxonomic markers. These findings highlight the functional and industrial potential of Tamarix and Agrimonia honeys as valuable food and nutraceutical products. Copyright
Agrimonia honey , Anticholinesterase activity , Antioxidant activity , Chemometric classification , Coumarin , Gallic acid , p-hydroxyphenylacetic acid , Phenolic profile , Tamarix honey , Tyrosinase inhibitory activity , Urease inhibitory activity
Text of the article Перейти на текст статьи
Al-Farabi Kazakh National University, Faculty of Chemistry and Chemical Technology, Almaty, 050040, Kazakhstan
Department of Chemical and Biochemical Engineering, Geology and Oil-Gas Business Institute Named After K. Turyssov, Satbayev University, Almaty, 050043, Kazakhstan
Mugla Sitki Kocman University, Faculty of Science, Department of Chemistry, Mentese-Mugla, 48121, Turkey
Bandırma Onyedi Eylül University, Susurluk Forest and Agriculture Vocational School, Department of Chemistry and Chemical Processing Technologies, Susurluk-Balıkesir, 10600, Turkey
Mugla Sitki Kocman University, Koycegiz Vocational School, Köyceğiz-Mugla, 48800, Turkey
Mugla Sitki Kocman University, Köyceğiz Vocational School of Health Services, Köyceğiz-Mugla, 48800, Turkey
Al-Farabi Kazakh National University
Department of Chemical and Biochemical Engineering
Mugla Sitki Kocman University
Bandırma Onyedi Eylül University
Mugla Sitki Kocman University
Mugla Sitki Kocman University
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026