Classification of buckwheat honey produced in Kazakhstan according to their biochemical ingredients and bioactivities by chemometric approach
Ongalbek D. Tokul-Ölmez Ö. Şahin B. Küçükaydın S. Aydoğmuş-Öztürk F. Sıcak Y. Yeskaliyeva B. Öztürk M.
1 September 2024Elsevier Ltd
Food Chemistry
2024#451
Herein, nineteen buckwheat honey samples collected from 19 stations of different ecological zones of Kazakhstan were analysed for their pollen density, physicochemical properties, chemical composition, antioxidant, anticholinesterase, tyrosinase inhibitory, and urease inhibitory activities with chemometric approaches. Twelve phenolic compounds and fumaric acid were identified using HPLC-DAD, and mainly fumaric, p-hydroxybenzoic, p-coumaric, trans-2-hydroxy cinnamic acids, and chrysin were detected in all samples. The honey samples collected from the Northern zone exhibited best antioxidant activity in lipid peroxidation inhibitory (IC50:8.65 ± 0.50 mg/mL), DPPH• (IC50:17.07 ± 1.49 mg/mL), ABTS•+ (IC50:8.90 ± 0.65 mg/mL), CUPRAC (A0.50:7.51 ± 0.30 mg/mL) and metal chelating assay (IC50:10.39 ± 0.71 mg/mL). In contrast, South-eastern zone samples indicated better acetylcholinesterase (55.57 ± 0.83%), butyrylcholinesterase (49.59 ± 1.09%), tyrosinase (44.40 ± 1.21%), and moderate urease (24.57 ± 0.33%) inhibitory activities at 20 mg/mL. The chemometric classification of nineteen buckwheat honey was performed using PCA and HCA techniques. Both were supported by correlation analysis. Thirteen compounds contributed significantly to the clustering of buckwheat honey based on geographical origin.
Antioxidant activity , Buckwheat honey , Chemometric classification , Enzyme inhibitory activity , HPLC-DAD , Phenolic composition
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Al-Farabi Kazakh National University, Faculty of Chemistry and Chemical Technology, Almaty, Kazakhstan
Mugla Sitki Kocman University, Faculty of Science, Department of Chemistry, Mentese-Mugla, 48121, Turkey
Bandırma Onyedi Eylül University, Susurluk Agriculture and Forest Vocational School, Department of Chemistry and Chemical Processing Technologies, Susurluk-Balıkesir, Turkey
Mugla Sitki Kocman University, Köyceğiz Vocational School of Health Services Koycegiz-Mugla, Turkey
Mugla Sitki Kocman University, Koycegiz Vocational School, Mugla, Turkey
Mugla Sitki Kocman University, Food Analysis Application and Research Center, Mugla, Turkey
Al-Farabi Kazakh National University
Mugla Sitki Kocman University
Bandırma Onyedi Eylül University
Mugla Sitki Kocman University
Mugla Sitki Kocman University
Mugla Sitki Kocman University
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