Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing
Olaifa K. Nikodinovic-Runic J. Glišić B. Boschetto F. Marin E. Segreto F. Marsili E.
1 September 2021Elsevier Ltd
Electrochimica Acta
2021#389
Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of antifungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.
Amphotericin B , Biofilm , Candida albicans , Complex Ag3 , Electroanalysis , Fluconazole
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Biofilm Laboratory, School of Engineering and Digital Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Nur-Sultan, 010000, Kazakhstan
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, Belgrade, 11000, Serbia
Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, Kragujevac, 34000, Serbia
Kyoto Institute of Technology, Matsugasaki, 606-8585, Kyoto, Japan
Department of Plastic, Reconstructive and Aesthetic Surgery, Biomedical Campus, Via A.Del Portillo 200, Rome, 00128, Italy
Biofilm Laboratory
Institute of Molecular Genetics and Genetic Engineering
Department of Chemistry
Kyoto Institute of Technology
Department of Plastic
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