Miscanthus × giganteus Rhizobacterial Community Responses to Zn and Oil Sludge Co-Contamination
Nurzhanova A. Boulygina E. Sungurtseva I. Mamirova A. Berzhanova R. Muratova A.
September 2025Multidisciplinary Digital Publishing Institute (MDPI)
Agronomy
2025#15Issue 9
Soil contamination in industrial areas often involves complex mixtures of contaminants, making remediation a significant challenge. Microbe-assisted phytoremediation offers a promising solution, yet its success depends on understanding interaction between plants, microorganisms, and contaminants in rhizosphere. This study examined the effects of organic (oil sludge) and inorganic (Zn) contaminants, applied individually and in combination, on the rhizosphere bacterial community of Miscanthus × giganteus Greef et Deu (M×g), with emphasis on strains exhibiting plant growth-promoting, hydrocarbon-degrading, and metal-tolerant traits. A one-season greenhouse experiment included soils spiked with Zn (1650 mg kg−1) and/or oil sludge (15 mL kg−1). Oil sludge exerted a stronger influence on the taxonomic structure of rhizobacterial communities than Zn, largely shaping the patterns observed under co-contamination. Zn exposure increased the relative abundance of Actinobacteriota, whereas oil sludge favoured Proteobacteriota. Both contaminants, individually and together, enhanced the proportion of Sphingomonadaceae. Across all treatments, taxa with potential plant-growth-promoting traits were present, while co-contaminated soil harboured microorganisms capable of hydrocarbon degradation, heavy metal tolerance, and plant growth promotion. These findings highlight the adaptive capacity of the M×g rhizobiome and support its application in phytoremediation. The isolation and characterisation of rhizosphere-associated strains provide basis for developing microbial bioagents to enhance biomass production and remediation efficiency in multi-contaminated environments.
Miscanthus × giganteus , oil sludge , rhizobacterial community , taxonomic structure , Zn
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Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty, 050040, Kazakhstan
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Marx, 76, 420012, Russian Federation
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Entuziastov 13, Saratov, 410049, Russian Federation
Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty, 050040, Kazakhstan
Institute of Plant Biology and Biotechnology
Institute of Fundamental Medicine and Biology
Institute of Biochemistry and Physiology of Plants and Microorganisms
Department of Biotechnology
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