In Vitro Approbation of Microbial Preparations to Shield Fruit Crops from Fire Blight: Physio-Biochemical Parameters


Nurzhanova A.A. Mamirova A. Mursaliyeva V. Nurmagambetova A.S. Zhumasheva Z. Turdiyev T. Kushnarenko S. Ismailova E.
June 2024Multidisciplinary Digital Publishing Institute (MDPI)

Plants
2024#13Issue 11

The need for the increasing geographical spread of fire blight (FB) affecting fruit crops to be addressed led to large-scale chemicalization of the environmental matrices and reduction of plant productivity. The current study aimed to assess the effects of novel biopreparations at different exposure durations on photosynthetic pigment content and antioxidant enzyme activity in leaves of apple and pear varieties with varying levels of resistance to FB. Biopreparations were formulated from a cultural broth containing Lacticaseibacillus paracasei M12 or Bacillus amyloliquefaciens MB40 isolated from apple trees’ phyllosphere. Aseptic leaves from blight-resistant (endemic Malus sieversii cv. KG10), moderately resistant (Pyrus pyraster cv. Wild), and susceptible (endangered Malus domestica cv. Aport and Pyrus communis cv. Shygys) varieties were employed. The impact of biopreparations on fruit crop antioxidant systems and photosynthetic apparatuses was investigated in vitro. Study results indicated that FB-resistant varieties exhibit enhanced adaptability and oxidative stress resistance compared to susceptible ones. Plant response to biopreparations varied based on the plant’s initial FB sensitivity and exposure duration. Indeed, biopreparations improved the adaptive response of the assimilation apparatus, protein synthesis, and catalase and superoxide dismutase activity in susceptible varieties, suggesting that biopreparations have the potential for future commercialization to manage FB in fruit crops.

antioxidant enzymes , biopreparation , fire blight , photosynthetic pigments , protein synthesis

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Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty, 050040, Kazakhstan
Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty, 050040, Kazakhstan
Scientific Production Centre of Microbiology and Virology, Bogenbai Batyr 105, Almaty, 050010, Kazakhstan

Institute of Plant Biology and Biotechnology
Department of Biotechnology
Scientific Production Centre of Microbiology and Virology

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