Antioxidant potential and phytochemical analysis of medicinal plants used for livestock treatment in Kazakhstan
Nurlybayeva K. Bekturova A. Kali A. Nurkenova A. Abikenova F. Amanbay B. Amantayeva A. Mukasheva G.
July 2024University of Guilan
Caspian Journal of Environmental Sciences
2024#22Issue 3653 - 662 pp.
Traditional veterinary practices in Kazakhstan rely heavily on medicinal plants for treating livestock diseases. However, scientific validation of these plants phytochemical composition and antioxidant properties remains limited. This researchs objective was to assess the antioxidant properties and examine the phytochemical composition of commonly used medicinal plants in Kazakhstan for livestock treatment: Glycyrrhiza uralensis, Ephedra equisetina, Rhodiola rosea, Hypericum perforatum, and Artemisia absinthium. Fresh samples of the selected plants were collected from local markets in Kazakhstan. Phytochemical screening was conducted using standard methods to detect tannins, flavonoids, alkaloids, saponins, and terpenoids. Total phenolic and flavonoid contents were determined spectrophotometrically. Antioxidant activity was assessed using DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays. To pinpoint and measure important phenolic components in the extracts exhibiting highest activity, High-Performance Liquid Chromatography (HPLC) examination was conducted. All five plants exhibited diverse phytochemical profiles, with flavonoids and terpenoids being ubiquitous. G. uralensis and R. rosea demonstrated the highest total phenolic (82.7 ± 3.5 and 75.9 ± 2.8 mg GAE/g, respectively) and flavonoid (45.3 ± 1.9 and 40.2 ± 1.7 mg QE/g, respectively) contents. These two species also showed the most potent antioxidant activities, with G. uralensis exhibiting the lowest IC50 value (42.8 ± 1.7 μg mL-1) in the DPPH assay and the highest FRAP value (1285.6 ± 52.3 μmol Fe(II)/g). The analysis revealed robust positive associations linking total phenolic content (r > 0.95, p < 0.01) and antioxidant activities. HPLC analysis revealed distinct phenolic profiles for G. uralensis and R. rosea, with high concentrations of rutin and chlorogenic acid, respectively. This research presents experimental corroboration, lending credence to the traditional use of these medicinal plants in livestock treatment, highlighting their rich phytochemical composition and potent antioxidant properties. G. uralensis and R. rosea emerged as particularly promising sources of natural antioxidants.
Antioxidants , Kazakhstan , Livestock , Medicinal plants , Phytochemicals
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Karaganda Buketov University, Street, Universitetskaya 28, Karaganda, Kazakhstan
L.N. Gumilyov Eurasian National University, Satpaev 2 Str, Astana, Kazakhstan
NP JSC «Karaganda Medical University», Department of Physiology, Gogol Street 40, Karaganda, Kazakhstan
Abai Kazakh National Pedagogical University, Dostyk 13, Almaty, Kazakhstan
Karaganda Buketov University
L.N. Gumilyov Eurasian National University
NP JSC «Karaganda Medical University»
Abai Kazakh National Pedagogical University
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