Plant extract of limonium gmelinii attenuates oxidative responses in neurons, astrocytes, and cerebral endothelial cells in vitro and improves motor functions of rats after middle cerebral artery occlusion
Nurkenov T. Tsoy A. Olzhayev F. Abzhanova E. Turgambayeva A. Zhussupova A. Avula B. Ross S. Aituarova A. Kassymova D. Zhusupova G. Shalakhmetova T. Tokay T. Lee J.C.-M. Askarova S.
November 2021MDPI
Antioxidants
2021#10Issue 11
There are numerous publications demonstrating that plant polyphenols can reduce oxi-dative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of L. gmelinii since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H2O2, and TNF‐α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P‐selectin expression, and activity of ERK1/2 were evaluated by quan-titative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF‐α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF‐α induced oxidative stress and triggered the accumulation of P‐selectin on the surface of the cells. In turn, pre‐treatment of the cells with the extract of L. gmelinii suppressed oxidative stress in all cell types and pro‐inflam-matory responses in astrocytes and CECs. In vivo, the treatment with L. gmelinii extract improved motor activity in rats with MCAO.
Antioxidants , Astrocytes , CECs , L. gmelinii , Neurons , Plant polyphenols
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Faculty of Biology and Biotechnology, Al‐Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Department of Biology, Institute of Natural Science and Geography, Abai Kazakh National Pedagogical University, Almaty, 050010, Kazakhstan
National Laboratory Astana, Nazarbayev University, Nur‐Sultan, 010000, Kazakhstan
School of Pharmacy, University of Mississippi, Oxford, 38677, MS, United States
School of Sciences and Humanities, Nazarbayev University, Nur‐Sultan, 010000, Kazakhstan
Department of Bioengineering, University of Illinois at Chicago, Chicago, 60612, IL, United States
Faculty of Biology and Biotechnology
Department of Biology
National Laboratory Astana
School of Pharmacy
School of Sciences and Humanities
Department of Bioengineering
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