Enhancing Rice Blast Resistance through Gene Pyramiding and Marker-Assisted Selection: Implications for Sustainable Agriculture and Environmental Impact
Mynbayeva D. Amirova A. Usenbekov B. Dubina E. Sartbayeva I. Zhunusbayeva Z. Yrgynbayeva S. Askhat K. Zeinalov Z.
1 December 2024Engineered Science Publisher
ES Energy and Environment
2024#26
Rice blast is the most harmful disease caused by Magnaporthe oryzae (M. oryzae), leading to yield loss. The aim of the work is to improve the resistance of rice to blast by introducing pyramid genes using conventional breeding and modern Marker Assisted Selection (MAS) techniques. Pyramiding is the combination of several resistance genes in one genotype, which improves the resistance of rice to pathogens. In this study, 7 pyramidal lines were identified with the introduction of a combination of 2 blast resistance genes (Pi-1 and Pi-ta) into 6 hybrids and 3 genes (Pi-1, Pi-33 and Pi-ta) into 1 line. Phytopathological testing of local rice cultivars and pyramidal lines showed that 3 out of 7 pyramidal lines were moderate resistant: 2 lines (F2 Bakanasski/7667 var. vulgaris and F2 Aisaule/7664 var. italica), containing 2 resistance genes (Pi-1 and Pita) and 1 line-F2 Aisaule/7689 var. zeravschanica, carrying 3 genes (Pi-1, Pi-33 and Pi-ta), while the local cultivars Bakanasski, Aisaule, and Aru, containing the blast resistance gene Pi-2, are susceptible. As a result, the blast resistance of rice was improved by creating pyramidal lines. Pyramided lines can be used for germplasm exchange and in rice breeding programs to improve blast resistance in rice.
Gene pyramiding , Marker assisted selection , Phytopathology , Rice Hybrid; Magnaporthe oryzae; Resistance
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Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040, Kazakhstan
Department of Molecular Biology and Genetics, Al-Farabi Kazakh National University, 71 Al-Farabi ave., Almaty, 050040, Kazakhstan
Kuban State Agrarian University, Krasnodar, 350044, Russian Federation
FSBSI “Federal Scientific Rice Centre”, Krasnodar, 350921, Russian Federation
Kazakh National Medical University named after S.D. Asfendiyarov, Tole Bi str., 94, Almaty, 050012, Kazakhstan
Faculty of Forestry, Wildlife And Environment, S. Seifullin Kazakh agrotechnical research university, Zhenis avenue 62, Astana city, 010011, Kazakhstan
Institute of Plant Biology and Biotechnology
Department of Molecular Biology and Genetics
Kuban State Agrarian University
FSBSI “Federal Scientific Rice Centre”
Kazakh National Medical University named after S.D. Asfendiyarov
Faculty of Forestry
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