Obtaining of adventitious roots culture of Allochrusa gypsophiloides: saponins-bearing endemic rare species
Mursaliyeva V. Algazy A. Satybaldiyeva D. Mukhanov T.
June 2023Springer
Plant Biotechnology Reports
2023#17Issue 3421 - 425 pp.
This study was conducted to establish adventitious roots (AR) culture from the original seeds of wild plants Allochrusa gypsophiloides (Regel) Schischk, a Central Asian rare species, whose roots are a rich source of oleanane-type triterpene saponins. The root explants from in vitro raised seedlings were cultured on liquid Murashige and Skoog medium (MS) with 1 mg/L indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA) under continuous agitation and total dark condition during a 45-day cycle. The AR induction, fresh (dry) root weight, growth index, and total saponins content in culture were evaluated. The high proliferative activity of AR on an auxin-containing medium resulted in accumulation and increased growth of AR relative to control, with a significant 3.5-fold excess on ½ MS with IBA. An increase in biomass AR on IBA and NAA medium correlated with the increased saponins content in their ethanol extracts compared to hormone-free. The excess of saponins in vitro on auxin-containing medium relative to in vivo roots averaged 138%, on control medium the saponins content was 1.5 timers lower. For the first time, the AR culture in vitro with a high growth index and high production of saponins for A. gypsophiloides was obtained.
Adventitious roots in vitro , Auxin , Growth index , Triterpene saponins , Turkestan soap root
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Institute of Plant Biology and Biotechnology, Ministry of Education and Science of the Republic of Kazakhstan, 45 Timiryazev St., Almaty, 050040, Kazakhstan
Institute of Plant Biology and Biotechnology
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